|T000008, K10 Tracer

T000008

K10 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

: 13.5

Z’: 0.82

: yes

Tracer titration

KD_app:

66 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: NLuc-AAK1

Reference: source

: 2.23

Z’: 0.83

: yes

Tracer titration

KD_app:

858 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

EC₅₀:

18563 µM

raw data

Comment: NLuc-ABL1(Q252H)

Reference: source

: 2.23

Z’: 0.93

: yes

Tracer titration

KD_app:

836 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

3 nM

raw data

Comment: NLuc-ABL2

Reference: source

: 6.1

Z’: 0.78

: yes

Tracer titration

KD_app:

9 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

25 nM

raw data

Comment: AURKA-NLuc

Reference: source

: 9.4

Z’: 0.6

: yes

Tracer titration

KD_app:

9 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: AURKB-NLuc

Reference: source

: 9.1

Z’: 0.71

: yes

Tracer titration

KD_app:

22 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: AURKC-NLuc

Reference: source

: 3.0

Z’: 0.91

: yes

Tracer titration

KD_app:

157 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

13 nM

raw data

Comment: AXL-NLuc

Reference: source

: 7.15

Z’: 0.78

: yes

Tracer titration

KD_app:

174 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

38 nM

raw data

Comment: NLuc-BMP2K

Reference: source

: 3.59

Z’: 0.91

: yes

Tracer titration

KD_app:

609 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

6 nM

raw data

Comment: BMX-NLuc

Reference: source

: 3.3

Z’: 0.85

: yes

Tracer titration

KD_app:

62 nM

raw data

Tracer displacement

i

Compound used for displacement:

PD-173955

EC₅₀:

38 nM

raw data

Comment: BRAF(V600E)-NLuc

Reference: source

: 5.16

Z’: 0.92

: yes

Tracer titration

KD_app:

518 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: NLuc-BRSK1

Reference: source

: 3.42

Z’: 0.88

: yes

Tracer titration

KD_app:

791 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

5 nM

raw data

Comment: NLuc-BRSK2

Reference: source

: 4.82

Z’: 0.94

: yes

Tracer titration

KD_app:

1053 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

15 nM

raw data

Comment: BTK-NLuc

: 2.7

Z’: 0.82

: yes

Tracer titration

KD_app:

720 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: BTK(C481S)-NLuc

Reference: source

: 4.9

Z’: 0.86

: yes

Tracer titration

KD_app:

308 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

6 nM

raw data

Comment: BTK(E41K)-NLuc

: 3.88

Z’: 0.56

: yes

Tracer titration

KD_app:

167 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: BTK(P190K)-NLuc

: 2.15

Z’: 0.34

: yes

Tracer titration

KD_app:

917 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

52 nM

raw data

Comment: NLuc-CAMK1

Reference: source

: 3.91

Z’: 0.7

: yes

Tracer titration

KD_app:

645 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

10 nM

raw data

Comment: NLuc-CAMK2A

Reference: source

: 5.54

Z’: 0.86

: yes

Tracer titration

KD_app:

452 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

24 nM

raw data

Comment: NLuc-CAMK2D

Reference: source

: 2.52

Z’: 0.89

: yes

Tracer titration

KD_app:

638 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

6 nM

raw data

Comment: CAMK2G-NLuc

Reference: source

: 2.97

Z’: 0.74

: yes

Tracer titration

KD_app:

551 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

94 nM

raw data

Comment: CDK1-NLuc + CycB1

Reference: source

: 3.03

Z’: 0.91

: yes

Tracer titration

KD_app:

485 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

91 nM

raw data

Comment: CDK1-NLuc + CycE1

Reference: source

: 3.23

Z’: 0.63

: yes

Tracer titration

KD_app:

663 nM

raw data

Tracer displacement

i

Compound used for displacement:

SNS-032

EC₅₀:

71 nM

raw data

Comment: CDK10-NLuc + CycL2

Reference: source

: 2.94

Z’: 0.9

: yes

Tracer titration

KD_app:

520 nM

raw data

Tracer displacement

i

Compound used for displacement:

SNS-032

EC₅₀:

941 nM

raw data

Comment: CDK14-NLuc + CycY

Reference: source

: 2.87

Z’: 0.92

: yes

Tracer titration

KD_app:

172 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

195 nM

raw data

Comment: NLuc-CDK15 + CycY

Reference: source

: 4.22

Z’: 0.9

: yes

Tracer titration

KD_app:

366 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

119 nM

raw data

Comment: CDK16-NLuc + CycY

Reference: source

: 5.66

Z’: 0.88

: yes

Tracer titration

KD_app:

457 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

260 nM

raw data

Comment: CDK17-NLuc + CycY

Reference: source

: 4.83

Z’: 0.79

: yes

Tracer titration

KD_app:

537 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

858 nM

raw data

Comment: CDK18-NLuc + CycY

Reference: source

: 6.96

Z’: 0.6

: yes

Tracer titration

KD_app:

364 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

1 µM

raw data

Comment: CDK2-NLuc + CycA1

Reference: source

: 7.07

Z’: 0.87

: yes

Tracer titration

KD_app:

647 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

516 nM

raw data

Comment: CDK2-NLuc + CycE1

Reference: source

: 6.55

Z’: 0.95

: yes

Tracer titration

KD_app:

422 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

2 µM

raw data

Comment: CDK2-NLuc

Reference: source

: 7.57

Z’: 0.74

: yes

Tracer titration

KD_app:

488 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

195 nM

raw data

Comment: NLuc-CDK20 + CycH

Reference: source

: 4.7

Z’: 0.93

: yes

Tracer titration

KD_app:

503 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

573 nM

raw data

Comment: CDK3-NLuc + CycE1

Reference: source

: 3.0

Z’: 0.79

: yes

Tracer titration

KD_app:

422 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

94 nM

raw data

Comment: NLuc-CDK4 + CycD1

Reference: source

: 4.33

Z’: 0.9

: yes

Tracer titration

KD_app:

312 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

136 nM

raw data

Comment: NLuc-CDK4 + CycD3

Reference: source

: 9.28

Z’: 0.82

: yes

Tracer titration

KD_app:

591 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

32 nM

raw data

Comment: CDK5-NLuc + CDK5R1

Reference: source

: 9.07

Z’: 0.86

: yes

Tracer titration

KD_app:

635 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

15 nM

raw data

Comment: CDK5-NLuc + CDK5R2

Reference: source

: 2.37

Z’: 0.85

: yes

Tracer titration

KD_app:

540 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

21 nM

raw data

Comment: NLuc-CDK6 + CycD1

Reference: source

: 3.05

Z’: 0.84

: yes

Tracer titration

KD_app:

793 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

EC₅₀:

212 nM

raw data

Comment: NLuc-CDK7 + pGEM

Reference: source

: 3.26

Z’: 0.89

: yes

Tracer titration

KD_app:

248 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

47 nM

raw data

Comment: NLuc-CDK9 + CycK

Reference: source

: 2.6

Z’: 0.74

: yes

Tracer titration

KD_app:

612 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

132 nM

raw data

Comment: NLuc-CDK9 + CycT1

Reference: source

: 2.23

Z’: 0.83

: yes

Tracer titration

KD_app:

290 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

8 nM

raw data

Comment: NLuc-CDKL1

Reference: source

: 7.46

Z’: 0.73

: yes

Tracer titration

KD_app:

363 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

3 nM

raw data

Comment: NLuc-CDKL2

Reference: source

: 2.28

Z’: 0.82

: yes

Tracer titration

KD_app:

327 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

2 nM

raw data

Comment: NLuc-CDKL3

Reference: source

: 3.69

Z’: 0.63

: yes

Tracer titration

KD_app:

355 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

1 nM

raw data

Comment: NLuc-CDKL5

Reference: source

: 2.01

Z’: 0.7

: yes

Tracer titration

KD_app:

817 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

12 nM

raw data

Comment: NLuc-CHEK1

Reference: source

: 2.02

Z’: 0.86

: yes

Tracer titration

KD_app:

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

906 nM

raw data

Comment: CHEK2-NLuc

Reference: source

: 2.63

Z’: 0.75

: yes

Tracer titration

KD_app:

823 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

236 nM

raw data

Comment: NLuc-CILK1

Reference: source

: 5.37

Z’: 0.92

: yes

Tracer titration

KD_app:

282 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: NLuc-CLK1

Reference: source

: 4.54

Z’: 0.95

: yes

Tracer titration

KD_app:

380 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

73 nM

raw data

Comment: CLK2-NLuc

Reference: source

: 3.18

Z’: 0.93

: yes

Tracer titration

KD_app:

134 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

39 nM

raw data

Comment: CLK4-NLuc

Reference: source

: 3.04

Z’: 0.86

: yes

Tracer titration

KD_app:

533 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

396 nM

raw data

Comment: NLuc-COQ8B

Reference: source

: 2.4

Z’: 0.6

: yes

Tracer titration

KD_app:

948 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

2 µM

raw data

Comment: NLuc-CSNK1A1L

Reference: source

: 2.25

Z’: 0.82

: yes

Tracer titration

KD_app:

794 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

1 µM

raw data

Comment: NLuc-CSNK1D

Reference: source

: 2.37

Z’: 0.78

: yes

Tracer titration

KD_app:

806 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

315 nM

raw data

Comment: NLuc-CSNK1G2

Reference: source

: 2.77

Z’: 0.87

: yes

Tracer titration

KD_app:

358 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

122 nM

raw data

Comment: CSNK2A1-NLuc

Reference: source

: 6.2

Z’: 0.9

: yes

Tracer titration

KD_app:

188 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

606 nM

raw data

Comment: CSNK2A2-NLuc

Reference: source

: 2.61

Z’: 0.95

: yes

Tracer titration

KD_app:

456 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

111 nM

raw data

Comment: NLuc-DAPK2

Reference: source

: 1.6

Z’: 0.6

: yes

Tracer titration

KD_app:

6460 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

50 nM

raw data

: 2.39

Z’: 0.84

: yes

Tracer titration

KD_app:

623 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

2 µM

raw data

Comment: DCLK3-NLuc

Reference: source

: 9.6

Z’: 0.88

: yes

Tracer titration

KD_app:

26 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

8 nM

raw data

: 2.15

Z’: 0.93

: yes

Tracer titration

KD_app:

858 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

10 nM

raw data

Comment: NLuc-DMPK

Reference: source

: 2.26

Z’: 0.82

: yes

Tracer titration

KD_app:

487 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

75 nM

raw data

Comment: NLuc-DYRK1A

Reference: source

: 2.73

Z’: 0.97

: yes

Tracer titration

KD_app:

309 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

35 nM

raw data

Comment: NLuc-DYRK1B

Reference: source

: 2.1

Z’: 0.83

: yes

Tracer titration

KD_app:

451 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

351 nM

raw data

Comment: DYRK2-NLuc

Reference: source

: 3.34

Z’: 0.96

: yes

Tracer titration

KD_app:

994 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 µM

raw data

Comment: NLuc-EIF2AK4 (Domain 2, 590-1001)

Reference: source

: 2.45

Z’: 0.93

: yes

Tracer titration

KD_app:

664 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

129 nM

raw data

Comment: EPHA1-NLuc

Reference: source

: 2.32

Z’: 0.74

: yes

Tracer titration

KD_app:

584 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

198 nM

raw data

Comment: EPHA4-NLuc

Reference: source

: 2.82

Z’: 0.81

: yes

Tracer titration

KD_app:

373 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

13 nM

raw data

Comment: EPHA6-NLuc

Reference: source

: 4.5

Z’: 0.97

: yes

Tracer titration

KD_app:

377 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

108 nM

raw data

Comment: EPHA7-NLuc

Reference: source

: 2.19

Z’: 0.87

: yes

Tracer titration

KD_app:

757 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: EPHB1-NLuc

Reference: source

: 2.27

Z’: 0.94

: yes

Tracer titration

KD_app:

775 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

94 nM

raw data

Comment: EPHB4-NLuc

Reference: source

: 2.61

Z’: 0.92

: yes

Tracer titration

KD_app:

241 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 µM

raw data

Comment: ERN1-NLuc

Reference: source

: 3.18

Z’: 0.84

: yes

Tracer titration

KD_app:

406 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

25 nM

raw data

Comment: ERN2-NLuc

Reference: source

: 2.67

Z’: 0.88

: yes

Tracer titration

KD_app:

446 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

170 nM

raw data

Comment: FER-NLuc

Reference: source

: 2.36

Z’: 0.7

: yes

Tracer titration

KD_app:

476 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

620 nM

raw data

Comment: FES-NLuc

Reference: source

: 3.9

Z’: 0.94

: yes

Tracer titration

KD_app:

232 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

387 nM

raw data

Comment: FGFR1-NLuc

Reference: source

: 2.9

Z’: 0.77

: yes

Tracer titration

KD_app:

138 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

311 nM

raw data

Comment: FGFR2-NLuc

: 2.54

Z’: 0.88

: yes

Tracer titration

KD_app:

205 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

2 µM

raw data

Comment: FGFR3-NLuc

Reference: source

: 2.58

Z’: 0.91

: yes

Tracer titration

KD_app:

158 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

2 µM

raw data

Comment: FGFR3(G697C)-NLuc

Reference: source

: 2.46

Z’: 0.65

: yes

Tracer titration

KD_app:

755 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

69 nM

raw data

Comment: FGFR4-NLuc

Reference: source

: 2.02

Z’: 0.91

: yes

Tracer titration

KD_app:

95 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

43 nM

raw data

Comment: FLT1-NLuc

Reference: source

: 2.57

Z’: 0.76

: yes

Tracer titration

KD_app:

130 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

17 nM

raw data

Comment: FLT3-NLuc

: 2.83

Z’: 0.76

: yes

Tracer titration

KD_app:

15 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

18 nM

raw data

Comment: FLT3(D835H)-NLuc

Reference: source

: 3.16

Z’: 0.61

: yes

Tracer titration

KD_app:

11 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

54 nM

raw data

Comment: FLT3(D835V)-NLuc

Reference: source

: 3.19

Z’: 0.63

: yes

Tracer titration

KD_app:

13 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

33 nM

raw data

Comment: FLT3(D835H)-NLuc

Reference: source

: 2.46

Z’: 0.58

: yes

Tracer titration

KD_app:

26 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

2 nM

raw data

Comment: FLT3(K663Q)-NLuc

Reference: source

: 3.2

Z’: 0.83

: yes

Tracer titration

KD_app:

33 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

7 nM

raw data

Comment: FLT3(N841I)-NLuc

Reference: source

: 2.7

Z’: 0.89

: yes

Tracer titration

KD_app:

34 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

2 nM

raw data

Comment: FLT3(R834Q)-NLuc

Reference: source

: 2.45

Z’: 0.93

: yes

Tracer titration

KD_app:

262 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

535 nM

raw data

Comment: FYN-NLuc

Reference: source

: 3.32

Z’: 0.73

: yes

Tracer titration

KD_app:

130 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

2 µM

raw data

Comment: FYN(Y531F)-NLuc

Reference: source

: 10.56

Z’: 0.64

: yes

Tracer titration

KD_app:

43 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

528 nM

raw data

Comment: NLuc-GAK

Reference: source

: 2.45

Z’: 0.86

: yes

Tracer titration

KD_app:

746 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

74 nM

raw data

Comment: NLuc-HIPK2

Reference: source

: 2.37

Z’: 0.55

: yes

Tracer titration

KD_app:

1044 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

190 nM

raw data

Comment: NLuc-HIPK3

Reference: source

: 11.23

Z’: 0.88

: yes

Tracer titration

KD_app:

366 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

5 µM

raw data

Comment: NLuc-HIPK4

Reference: source

: 2.0

Z’: 0.63

: yes

Tracer titration

KD_app:

179 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

6 µM

raw data

Comment: IGF1R-NLuc

Reference: source

: 3.77

Z’: 0.75

: yes

Tracer titration

KD_app:

525 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

239 nM

raw data

Comment: NLuc-IKBKE

Reference: source

: 2.61

Z’: 0.73

: yes

Tracer titration

KD_app:

189 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 µM

raw data

Comment: INSR-NLuc

Reference: source

: 3.65

Z’: 0.82

: yes

Tracer titration

KD_app:

20 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

8 nM

raw data

Comment: NLuc-IRAK3

Reference: source

: 6.04

Z’: 0.91

: yes

Tracer titration

KD_app:

154 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

17 nM

raw data

Comment: IRAK4-NLuc

Reference: source

: 3.42

Z’: 0.88

: yes

Tracer titration

KD_app:

67 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 µM

raw data

Comment: NLuc-ITK

Reference: source

: 2.49

Z’: 0.22

: yes

Tracer titration

KD_app:

846 nM

raw data

Tracer displacement

i

Compound used for displacement:

Ruxolitinib

EC₅₀:

246 nM

raw data

Comment: JAK2-NLuc

Reference: source

: 3.75

Z’: 0.77

: yes

Tracer titration

KD_app:

186 nM

raw data

Tracer displacement

i

Compound used for displacement:

Ruxolitinib

EC₅₀:

6 nM

raw data

Comment: JAK2(V617F)-NLuc

Reference: source

: 2.49

Z’: 0.85

: yes

Tracer titration

KD_app:

566 nM

raw data

Tracer displacement

i

Compound used for displacement:

Ruxolitinib

EC₅₀:

23 nM

raw data

Comment: JAK2 (JH1 domain, 840–1132)

Reference: source

: 2.99

Z’: 0.9

: yes

Tracer titration

KD_app:

419 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

35 nM

raw data

Comment: JAK3-NLuc

Reference: source

: 2.06

Z’: 0.82

: yes

Tracer titration

KD_app:

324 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

89 nM

raw data

Comment: KIT(A829P)-NLuc

Reference: source

: 2.15

Z’: 0.8

: yes

Tracer titration

KD_app:

272 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

67 nM

raw data

Comment: KIT(D816H)-NLuc

Reference: source

: 3.35

Z’: 0.91

: yes

Tracer titration

KD_app:

263 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

1 µM

raw data

Comment: KIT(D816V)-NLuc

Reference: source

: 2.27

Z’: 0.71

: yes

Tracer titration

KD_app:

228 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

44 nM

raw data

Comment: KIT(V559D)-NLuc

Reference: source

: 3.93

Z’: 0.78

: yes

Tracer titration

KD_app:

99 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

754 nM

raw data

Comment: LATS1-NLuc

Reference: source

: 3.52

Z’: 0.77

: yes

Tracer titration

KD_app:

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

642 nM

raw data

Comment: LATS2-NLuc

Reference: source

: 2.48

Z’: 0.88

: yes

Tracer titration

KD_app:

364 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

224 nM

raw data

Comment: LCK-NLuc

Reference: source

: 4.22

Z’: 0.76

: yes

Tracer titration

KD_app:

992 nM

raw data

Tracer displacement

i

Compound used for displacement:

HG-9-91-01

EC₅₀:

18 nM

raw data

Comment: LIMK1-NLuc

Reference: source

: 5.22

Z’: 0.94

: yes

Tracer titration

KD_app:

468 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

179 nM

raw data

Comment: LIMK2-NLuc

Reference: source

: 2.7

Z’: 0.84

: yes

Tracer titration

KD_app:

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

4 nM

raw data

Comment: LRRK2-NLuc

: 2.09

Z’: 0.73

: yes

Tracer titration

KD_app:

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 nM

raw data

Comment: LRRK2(G2019S)-NLuc

Reference: source

: 3.93

Z’: 0.95

: yes

T000008

K10 Tracer

Properties

References

Experimental

Target: AAK1 63 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: ABL1 1000 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: ABL2 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: AURKA 8 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: AURKB 8 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: AURKC 16 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: AXL 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BMP2K 130 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BMX 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BRAF 130 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BRSK1 250 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BRSK2 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BTK 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BTK 1000 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BTK 130 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: BTK 130 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: CAMK1 1000 nM Assay: NanoBRET Level: expert

Tracer titration

Tracer displacement

Target: CAMK2A 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: CAMK2D 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: CAMK2G 130 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: CDK1 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: CDK1 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: CDK10 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: CDK14 500 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement

Target: CDK15 250 nM Assay: NanoBRET Level: robust

Tracer titration

Tracer displacement