|T000008, K10 Tracer

Compound Image

T000008

K10 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

: 13.5

Z’: 0.82

: yes

Tracer titration

KD_app:

66 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: NLuc-AAK1

Reference: source

: 2.23

Z’: 0.83

: yes

Tracer titration

KD_app:

858 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

EC₅₀:

18563 µM

raw data

Comment: NLuc-ABL1(Q252H)

Reference: source

: 2.23

Z’: 0.93

: yes

Tracer titration

KD_app:

836 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

3 nM

raw data

Comment: NLuc-ABL2

Reference: source

: 6.1

Z’: 0.78

: yes

Tracer titration

KD_app:

9 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

25 nM

raw data

Comment: AURKA-NLuc

Reference: source

: 9.4

Z’: 0.6

: yes

Tracer titration

KD_app:

9 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: AURKB-NLuc

Reference: source

: 9.1

Z’: 0.71

: yes

Tracer titration

KD_app:

22 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: AURKC-NLuc

Reference: source

: 3.0

Z’: 0.91

: yes

Tracer titration

KD_app:

157 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

13 nM

raw data

Comment: AXL-NLuc

Reference: source

: 7.15

Z’: 0.78

: yes

Tracer titration

KD_app:

174 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

38 nM

raw data

Comment: NLuc-BMP2K

Reference: source

: 3.59

Z’: 0.91

: yes

Tracer titration

KD_app:

609 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

6 nM

raw data

Comment: BMX-NLuc

Reference: source

: 3.3

Z’: 0.85

: yes

Tracer titration

KD_app:

62 nM

raw data

Tracer displacement

i

Compound used for displacement:

PD-173955

EC₅₀:

38 nM

raw data

Comment: BRAF(V600E)-NLuc

Reference: source

: 5.16

Z’: 0.92

: yes

Tracer titration

KD_app:

518 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: NLuc-BRSK1

Reference: source

: 3.42

Z’: 0.88

: yes

Tracer titration

KD_app:

791 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

5 nM

raw data

Comment: NLuc-BRSK2

Reference: source

: 4.82

Z’: 0.94

: yes

Tracer titration

KD_app:

1053 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

15 nM

raw data

Comment: BTK-NLuc

: 2.7

Z’: 0.82

: yes

Tracer titration

KD_app:

720 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: BTK(C481S)-NLuc

Reference: source

: 4.9

Z’: 0.86

: yes

Tracer titration

KD_app:

308 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

6 nM

raw data

Comment: BTK(E41K)-NLuc

: 3.88

Z’: 0.56

: yes

Tracer titration

KD_app:

167 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: BTK(P190K)-NLuc

: 2.15

Z’: 0.34

: yes

Tracer titration

KD_app:

917 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

52 nM

raw data

Comment: NLuc-CAMK1

Reference: source

: 3.91

Z’: 0.7

: yes

Tracer titration

KD_app:

645 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

10 nM

raw data

Comment: NLuc-CAMK2A

Reference: source

: 5.54

Z’: 0.86

: yes

Tracer titration

KD_app:

452 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

24 nM

raw data

Comment: NLuc-CAMK2D

Reference: source

: 2.52

Z’: 0.89

: yes

Tracer titration

KD_app:

638 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

6 nM

raw data

Comment: CAMK2G-NLuc

Reference: source

: 2.97

Z’: 0.74

: yes

Tracer titration

KD_app:

551 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

94 nM

raw data

Comment: CDK1-NLuc + CycB1

Reference: source

: 3.03

Z’: 0.91

: yes

Tracer titration

KD_app:

485 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

91 nM

raw data

Comment: CDK1-NLuc + CycE1

Reference: source

: 3.23

Z’: 0.63

: yes

Tracer titration

KD_app:

663 nM

raw data

Tracer displacement

i

Compound used for displacement:

SNS-032

EC₅₀:

71 nM

raw data

Comment: CDK10-NLuc + CycL2

Reference: source

: 2.94

Z’: 0.9

: yes

Tracer titration

KD_app:

520 nM

raw data

Tracer displacement

i

Compound used for displacement:

SNS-032

EC₅₀:

941 nM

raw data

Comment: CDK14-NLuc + CycY

Reference: source

: 2.87

Z’: 0.92

: yes

Tracer titration

KD_app:

172 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

195 nM

raw data

Comment: NLuc-CDK15 + CycY

Reference: source

: 4.22

Z’: 0.9

: yes

Tracer titration

KD_app:

366 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

119 nM

raw data

Comment: CDK16-NLuc + CycY

Reference: source

: 5.66

Z’: 0.88

: yes

Tracer titration

KD_app:

457 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

260 nM

raw data

Comment: CDK17-NLuc + CycY

Reference: source

: 4.83

Z’: 0.79

: yes

Tracer titration

KD_app:

537 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

858 nM

raw data

Comment: CDK18-NLuc + CycY

Reference: source

: 6.96

Z’: 0.6

: yes

Tracer titration

KD_app:

364 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

1 µM

raw data

Comment: CDK2-NLuc + CycA1

Reference: source

: 7.07

Z’: 0.87

: yes

Tracer titration

KD_app:

647 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

516 nM

raw data

Comment: CDK2-NLuc + CycE1

Reference: source

: 6.55

Z’: 0.95

: yes

Tracer titration

KD_app:

422 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

2 µM

raw data

Comment: CDK2-NLuc

Reference: source

: 7.57

Z’: 0.74

: yes

Tracer titration

KD_app:

488 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

195 nM

raw data

Comment: NLuc-CDK20 + CycH

Reference: source

: 4.7

Z’: 0.93

: yes

Tracer titration

KD_app:

503 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

EC₅₀:

573 nM

raw data

Comment: CDK3-NLuc + CycE1

Reference: source

: 3.0

Z’: 0.79

: yes

Tracer titration

KD_app:

422 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

94 nM

raw data

Comment: NLuc-CDK4 + CycD1

Reference: source

: 4.33

Z’: 0.9

: yes

Tracer titration

KD_app:

312 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

136 nM

raw data

Comment: NLuc-CDK4 + CycD3

Reference: source

: 9.28

Z’: 0.82

: yes

Tracer titration

KD_app:

591 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

32 nM

raw data

Comment: CDK5-NLuc + CDK5R1

Reference: source

: 9.07

Z’: 0.86

: yes

Tracer titration

KD_app:

635 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

15 nM

raw data

Comment: CDK5-NLuc + CDK5R2

Reference: source

: 2.37

Z’: 0.85

: yes

Tracer titration

KD_app:

540 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

21 nM

raw data

Comment: NLuc-CDK6 + CycD1

Reference: source

: 3.05

Z’: 0.84

: yes

Tracer titration

KD_app:

793 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

EC₅₀:

212 nM

raw data

Comment: NLuc-CDK7 + pGEM

Reference: source

: 3.26

Z’: 0.89

: yes

Tracer titration

KD_app:

248 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

47 nM

raw data

Comment: NLuc-CDK9 + CycK

Reference: source

: 2.6

Z’: 0.74

: yes

Tracer titration

KD_app:

612 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

132 nM

raw data

Comment: NLuc-CDK9 + CycT1

Reference: source

: 2.23

Z’: 0.83

: yes

Tracer titration

KD_app:

290 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

8 nM

raw data

Comment: NLuc-CDKL1

Reference: source

: 7.46

Z’: 0.73

: yes

Tracer titration

KD_app:

363 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

3 nM

raw data

Comment: NLuc-CDKL2

Reference: source

: 2.28

Z’: 0.82

: yes

Tracer titration

KD_app:

327 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

2 nM

raw data

Comment: NLuc-CDKL3

Reference: source

: 3.69

Z’: 0.63

: yes

Tracer titration

KD_app:

355 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

1 nM

raw data

Comment: NLuc-CDKL5

Reference: source

: 2.01

Z’: 0.7

: yes

Tracer titration

KD_app:

817 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

12 nM

raw data

Comment: NLuc-CHEK1

Reference: source

: 2.02

Z’: 0.86

: yes

Tracer titration

KD_app:

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

906 nM

raw data

Comment: CHEK2-NLuc

Reference: source

: 2.63

Z’: 0.75

: yes

Tracer titration

KD_app:

823 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

236 nM

raw data

Comment: NLuc-CILK1

Reference: source

: 5.37

Z’: 0.92

: yes

Tracer titration

KD_app:

282 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: NLuc-CLK1

Reference: source

: 4.54

Z’: 0.95

: yes

Tracer titration

KD_app:

380 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

73 nM

raw data

Comment: CLK2-NLuc

Reference: source

: 3.18

Z’: 0.93

: yes

Tracer titration

KD_app:

134 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

39 nM

raw data

Comment: CLK4-NLuc

Reference: source

: 3.04

Z’: 0.86

: yes

Tracer titration

KD_app:

533 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

396 nM

raw data

Comment: NLuc-COQ8B

Reference: source

: 2.4

Z’: 0.6

: yes

Tracer titration

KD_app:

948 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

2 µM

raw data

Comment: NLuc-CSNK1A1L

Reference: source

: 2.25

Z’: 0.82

: yes

Tracer titration

KD_app:

794 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

1 µM

raw data

Comment: NLuc-CSNK1D

Reference: source

: 2.37

Z’: 0.78

: yes

Tracer titration

KD_app:

806 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

315 nM

raw data

Comment: NLuc-CSNK1G2

Reference: source

: 2.77

Z’: 0.87

: yes

Tracer titration

KD_app:

358 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

122 nM

raw data

Comment: CSNK2A1-NLuc

Reference: source

: 6.2

Z’: 0.9

: yes

Tracer titration

KD_app:

188 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

606 nM

raw data

Comment: CSNK2A2-NLuc

Reference: source

: 2.61

Z’: 0.95

: yes

Tracer titration

KD_app:

456 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

111 nM

raw data

Comment: NLuc-DAPK2

Reference: source

: 1.6

Z’: 0.6

: yes

Tracer titration

KD_app:

6460 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

50 nM

raw data

: 2.39

Z’: 0.84

: yes

Tracer titration

KD_app:

623 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

2 µM

raw data

Comment: DCLK3-NLuc

Reference: source

: 9.6

Z’: 0.88

: yes

Tracer titration

KD_app:

26 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

8 nM

raw data

: 2.15

Z’: 0.93

: yes

Tracer titration

KD_app:

858 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

10 nM

raw data

Comment: NLuc-DMPK

Reference: source

: 2.26

Z’: 0.82

: yes

Tracer titration

KD_app:

487 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

75 nM

raw data

Comment: NLuc-DYRK1A

Reference: source

: 2.73

Z’: 0.97

: yes

Tracer titration

KD_app:

309 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

35 nM

raw data

Comment: NLuc-DYRK1B

Reference: source

: 2.1

Z’: 0.83

: yes

Tracer titration

KD_app:

451 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

351 nM

raw data

Comment: DYRK2-NLuc

Reference: source

: 3.34

Z’: 0.96

: yes

Tracer titration

KD_app:

994 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 µM

raw data

Comment: NLuc-EIF2AK4 (Domain 2, 590-1001)

Reference: source

: 2.45

Z’: 0.93

: yes

Tracer titration

KD_app:

664 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

129 nM

raw data

Comment: EPHA1-NLuc

Reference: source

: 2.32

Z’: 0.74

: yes

Tracer titration

KD_app:

584 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

198 nM

raw data

Comment: EPHA4-NLuc

Reference: source

: 2.82

Z’: 0.81

: yes

Tracer titration

KD_app:

373 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

13 nM

raw data

Comment: EPHA6-NLuc

Reference: source

: 4.5

Z’: 0.97

: yes

Tracer titration

KD_app:

377 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

108 nM

raw data

Comment: EPHA7-NLuc

Reference: source

: 2.19

Z’: 0.87

: yes

Tracer titration

KD_app:

757 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: EPHB1-NLuc

Reference: source

: 2.27

Z’: 0.94

: yes

Tracer titration

KD_app:

775 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

94 nM

raw data

Comment: EPHB4-NLuc

Reference: source

: 2.61

Z’: 0.92

: yes

Tracer titration

KD_app:

241 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 µM

raw data

Comment: ERN1-NLuc

Reference: source

: 3.18

Z’: 0.84

: yes

Tracer titration

KD_app:

406 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

25 nM

raw data

Comment: ERN2-NLuc

Reference: source

: 2.67

Z’: 0.88

: yes

Tracer titration

KD_app:

446 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

170 nM

raw data

Comment: FER-NLuc

Reference: source

: 2.36

Z’: 0.7

: yes

Tracer titration

KD_app:

476 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

620 nM

raw data

Comment: FES-NLuc

Reference: source

: 3.9

Z’: 0.94

: yes

Tracer titration

KD_app:

232 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

387 nM

raw data

Comment: FGFR1-NLuc

Reference: source

: 2.9

Z’: 0.77

: yes

Tracer titration

KD_app:

138 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

311 nM

raw data

Comment: FGFR2-NLuc

: 2.54

Z’: 0.88

: yes

Tracer titration

KD_app:

205 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

2 µM

raw data

Comment: FGFR3-NLuc

Reference: source

: 2.58

Z’: 0.91

: yes

Tracer titration

KD_app:

158 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

2 µM

raw data

Comment: FGFR3(G697C)-NLuc

Reference: source

: 2.46

Z’: 0.65

: yes

Tracer titration

KD_app:

755 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

69 nM

raw data

Comment: FGFR4-NLuc

Reference: source

: 2.02

Z’: 0.91

: yes

Tracer titration

KD_app:

95 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

43 nM

raw data

Comment: FLT1-NLuc

Reference: source

: 2.57

Z’: 0.76

: yes

Tracer titration

KD_app:

130 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

17 nM

raw data

Comment: FLT3-NLuc

: 2.83

Z’: 0.76

: yes

Tracer titration

KD_app:

15 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

18 nM

raw data

Comment: FLT3(D835H)-NLuc

Reference: source

: 3.16

Z’: 0.61

: yes

Tracer titration

KD_app:

11 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

54 nM

raw data

Comment: FLT3(D835V)-NLuc

Reference: source

: 3.19

Z’: 0.63

: yes

Tracer titration

KD_app:

13 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

33 nM

raw data

Comment: FLT3(D835H)-NLuc

Reference: source

: 2.46

Z’: 0.58

: yes

Tracer titration

KD_app:

26 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

2 nM

raw data

Comment: FLT3(K663Q)-NLuc

Reference: source

: 3.2

Z’: 0.83

: yes

Tracer titration

KD_app:

33 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

7 nM

raw data

Comment: FLT3(N841I)-NLuc

Reference: source

: 2.7

Z’: 0.89

: yes

Tracer titration

KD_app:

34 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

2 nM

raw data

Comment: FLT3(R834Q)-NLuc

Reference: source

: 2.45

Z’: 0.93

: yes

Tracer titration

KD_app:

262 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

535 nM

raw data

Comment: FYN-NLuc

Reference: source

: 3.32

Z’: 0.73

: yes

Tracer titration

KD_app:

130 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

2 µM

raw data

Comment: FYN(Y531F)-NLuc

Reference: source

: 10.56

Z’: 0.64

: yes

Tracer titration

KD_app:

43 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

528 nM

raw data

Comment: NLuc-GAK

Reference: source

: 2.45

Z’: 0.86

: yes

Tracer titration

KD_app:

746 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

74 nM

raw data

Comment: NLuc-HIPK2

Reference: source

: 2.37

Z’: 0.55

: yes

Tracer titration

KD_app:

1044 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

190 nM

raw data

Comment: NLuc-HIPK3

Reference: source

: 11.23

Z’: 0.88

: yes

Tracer titration

KD_app:

366 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

5 µM

raw data

Comment: NLuc-HIPK4

Reference: source

: 2.0

Z’: 0.63

: yes

Tracer titration

KD_app:

179 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

6 µM

raw data

Comment: IGF1R-NLuc

Reference: source

: 3.77

Z’: 0.75

: yes

Tracer titration

KD_app:

525 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

239 nM

raw data

Comment: NLuc-IKBKE

Reference: source

: 2.61

Z’: 0.73

: yes

Tracer titration

KD_app:

189 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 µM

raw data

Comment: INSR-NLuc

Reference: source

: 3.65

Z’: 0.82

: yes

Tracer titration

KD_app:

20 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

8 nM

raw data

Comment: NLuc-IRAK3

Reference: source

: 6.04

Z’: 0.91

: yes

Tracer titration

KD_app:

154 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

17 nM

raw data

Comment: IRAK4-NLuc

Reference: source

: 3.42

Z’: 0.88

: yes

Tracer titration

KD_app:

67 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 µM

raw data

Comment: NLuc-ITK

Reference: source

: 2.49

Z’: 0.22

: yes

Tracer titration

KD_app:

846 nM

raw data

Tracer displacement

i

Compound used for displacement:

Ruxolitinib

EC₅₀:

246 nM

raw data

Comment: JAK2-NLuc

Reference: source

: 3.75

Z’: 0.77

: yes

Tracer titration

KD_app:

186 nM

raw data

Tracer displacement

i

Compound used for displacement:

Ruxolitinib

EC₅₀:

6 nM

raw data

Comment: JAK2(V617F)-NLuc

Reference: source

: 2.49

Z’: 0.85

: yes

Tracer titration

KD_app:

566 nM

raw data

Tracer displacement

i

Compound used for displacement:

Ruxolitinib

EC₅₀:

23 nM

raw data

Comment: JAK2 (JH1 domain, 840–1132)

Reference: source

: 2.99

Z’: 0.9

: yes

Tracer titration

KD_app:

419 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

35 nM

raw data

Comment: JAK3-NLuc

Reference: source

: 2.06

Z’: 0.82

: yes

Tracer titration

KD_app:

324 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

89 nM

raw data

Comment: KIT(A829P)-NLuc

Reference: source

: 2.15

Z’: 0.8

: yes

Tracer titration

KD_app:

272 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

67 nM

raw data

Comment: KIT(D816H)-NLuc

Reference: source

: 3.35

Z’: 0.91

: yes

Tracer titration

KD_app:

263 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

1 µM

raw data

Comment: KIT(D816V)-NLuc

Reference: source

: 2.27

Z’: 0.71

: yes

Tracer titration

KD_app:

228 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

44 nM

raw data

Comment: KIT(V559D)-NLuc

Reference: source

: 3.93

Z’: 0.78

: yes

Tracer titration

KD_app:

99 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

754 nM

raw data

Comment: LATS1-NLuc

Reference: source

: 3.52

Z’: 0.77

: yes

Tracer titration

KD_app:

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

642 nM

raw data

Comment: LATS2-NLuc

Reference: source

: 2.48

Z’: 0.88

: yes

Tracer titration

KD_app:

364 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

224 nM

raw data

Comment: LCK-NLuc

Reference: source

: 4.22

Z’: 0.76

: yes

Tracer titration

KD_app:

992 nM

raw data

Tracer displacement

i

Compound used for displacement:

HG-9-91-01

EC₅₀:

18 nM

raw data

Comment: LIMK1-NLuc

Reference: source

: 5.22

Z’: 0.94

: yes

Tracer titration

KD_app:

468 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

179 nM

raw data

Comment: LIMK2-NLuc

Reference: source

: 2.7

Z’: 0.84

: yes

Tracer titration

KD_app:

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

4 nM

raw data

Comment: LRRK2-NLuc

: 2.09

Z’: 0.73

: yes

Tracer titration

KD_app:

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 nM

raw data

Comment: LRRK2(G2019S)-NLuc

Reference: source

: 3.93

Z’: 0.95

: yes

Tracer titration

KD_app:

136 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

6 nM

raw data

Comment: LTK-NLuc

Reference: source

: 4.05

Z’: 0.93

: yes

Tracer titration

KD_app:

159 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

456 nM

raw data

Comment: NLuc-MAP3K10

Reference: source

: 4.28

Z’: 0.91

: yes

Tracer titration

KD_app:

175 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

20 nM

raw data

Comment: NLuc-MAP3K11

Reference: source

: 2.72

Z’: 0.94

: yes

Tracer titration

KD_app:

350 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

1 µM

raw data

Comment: NLuc-MAP3K12

Reference: source

: 2.12

Z’: 0.68

: yes

Tracer titration

KD_app:

77 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

305 nM

raw data

Comment: MAP3K19-NLuc

Reference: source

: 2.25

Z’: 0.89

: yes

Tracer titration

KD_app:

248 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

2 µM

raw data

Comment: MAP3K2-NLuc

Reference: source

: 2.6

Z’: 0.59

: yes

Tracer titration

KD_app:

434 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

114 nM

raw data

Comment: NLuc-MAP3K20

Reference: source

: 2.3

Z’: 0.8

: yes

Tracer titration

KD_app:

524 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

1 µM

raw data

Comment: NLuc-MAP3K21

Reference: source

: 2.28

Z’: 0.82

: yes

Tracer titration

KD_app:

318 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

EC₅₀:

2 µM

raw data

Comment: MAP3K3-NLuc

Reference: source

: 3.93

Z’: 0.89

: yes

Tracer titration

KD_app:

767 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

84 nM

raw data

Comment: MAP3K4-NLuc

Reference: source

: 2.63

Z’: 0.79

: yes

Tracer titration

KD_app:

146 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

10 nM

raw data

Comment: NLuc-MAP3K9

Reference: source

: 4.15

Z’: 0.9

: yes

Tracer titration

KD_app:

244 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

186 nM

raw data

Comment: NLuc-MAP4K1

Reference: source

: 3.13

Z’: 0.88

: yes

Tracer titration

KD_app:

116 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

8 nM

raw data

Comment: NLuc-MAP4K2

Reference: source

: 4.41

Z’: 0.8

: yes

Tracer titration

KD_app:

554 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

113 nM

raw data

Comment: NLuc-MAP4K3

Reference: source

: 2.38

Z’: 0.66

: yes

Tracer titration

KD_app:

2007 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

118 nM

raw data

Comment: MAP4K5-NLuc

Reference: source

: 2.45

Z’: 0.89

: yes

Tracer titration

KD_app:

193 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

235 nM

raw data

Comment: MAPK10-NLuc

Reference: source

: 4.04

Z’: 0.72

: yes

Tracer titration

KD_app:

806 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

1 µM

raw data

Comment: NLuc-MAPK11

Reference: source

: 3.19

Z’: 0.81

: yes

Tracer titration

KD_app:

559 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

136 nM

raw data

Comment: MAPK14-NLuc

Reference: source

: 5.98

Z’: 0.95

: yes

Tracer titration

KD_app:

263 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

399 nM

raw data

Comment: MAPK14(T106M)-NLuc

Reference: source

: 7.64

Z’: 0.74

: yes

Tracer titration

KD_app:

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

TMLH98

EC₅₀:

118 nM

raw data

Comment: NLuc-MAPK15

: 17.93

Z’: 0.75

: yes

Tracer titration

KD_app:

60 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

14 nM

raw data

Comment: NLuc-MAPK4

Reference: source

: 11.68

Z’: 0.83

: yes

Tracer titration

KD_app:

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

48 nM

raw data

Comment: NLuc-MAPK6

Reference: source

: 3.75

Z’: 0.7

: yes

Tracer titration

KD_app:

174 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

111 nM

raw data

Comment: NLuc-MAPK8

Reference: source

: 5.02

Z’: 0.78

: yes

Tracer titration

KD_app:

217 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

514 nM

raw data

Comment: NLuc-MAPK9

Reference: source

: 3.54

Z’: 0.94

: yes

Tracer titration

KD_app:

480 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

1 nM

raw data

Comment: NLuc-MARK2

Reference: source

: 2.44

Z’: 0.82

: yes

Tracer titration

KD_app:

1081 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 nM

raw data

Comment: NLuc-MARK3

Reference: source

: 3.15

Z’: 0.87

: yes

Tracer titration

KD_app:

295 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: NLuc-MARK4

: 2.13

Z’: 0.93

: yes

Tracer titration

KD_app:

444 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

388 nM

raw data

Comment: NLuc-MAST3

Reference: source

: 2.25

Z’: 0.81

: yes

Tracer titration

KD_app:

412 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

542 nM

raw data

Comment: NLuc-MAST4

Reference: source

: 2.62

Z’: 0.91

: yes

Tracer titration

KD_app:

1911 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

373 nM

raw data

Comment: NLuc-MELK

Reference: source

: 3.18

Z’: 0.85

: yes

Tracer titration

KD_app:

584 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

357 nM

raw data

Comment: NLuc-MELK(T460M)

Reference: source

: 2.05

Z’: 0.47

: yes

Tracer titration

KD_app:

125 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

28 nM

raw data

Comment: MERTK-NLuc

Reference: source

: 2.14

Z’: 0.93

: yes

Tracer titration

KD_app:

102 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

7 nM

raw data

Comment: MERTK(A708S)

Reference: source

: 3.23

Z’: 0.88

: yes

Tracer titration

KD_app:

543 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

6 nM

raw data

Comment: MET-NLuc

Reference: source

: 4.4

Z’: 0.91

: yes

Tracer titration

KD_app:

393 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

35 nM

raw data

Comment: MET(D1228H)-NLuc

Reference: source

: 5.25

Z’: 0.84

: yes

Tracer titration

KD_app:

355 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

26 nM

raw data

Comment: MET(D1228N)-NLuc

Reference: source

: 6.0

Z’: 0.95

: yes

Tracer titration

KD_app:

341 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

156 nM

raw data

Comment: MET(F1200I)-NLuc

Reference: source

: 5.45

Z’: 0.93

: yes

Tracer titration

KD_app:

243 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

9 nM

raw data

Comment: MET(M1250T)-NLuc

Reference: source

: 4.03

Z’: 0.8

: yes

Tracer titration

KD_app:

353 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

14 nM

raw data

Comment: MET(P991S)-NLuc

Reference: source

: 3.53

Z’: 0.84

: yes

Tracer titration

KD_app:

484 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

11 nM

raw data

Comment: MET(T1173I)-NLuc

Reference: source

: 3.86

Z’: 0.84

: yes

Tracer titration

KD_app:

394 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

4 nM

raw data

Comment: MET(T992I)-NLuc

Reference: source

: 5.87

Z’: 0.88

: yes

Tracer titration

KD_app:

364 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

1 nM

raw data

Comment: MET(V1092I)-NLuc

Reference: source

: 5.5

Z’: 0.78

: yes

Tracer titration

KD_app:

364 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

9 nM

raw data

Comment: MET(Y1230A)-NLuc

Reference: source

: 4.5

Z’: 0.72

: yes

Tracer titration

KD_app:

460 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

4 nM

raw data

Comment: MET(Y1230C)-NLuc

Reference: source

: 4.55

Z’: 0.9

: yes

Tracer titration

KD_app:

312 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

8 nM

raw data

Comment: MET(Y1230D)-NLuc

Reference: source

: 5.9

Z’: 0.85

: yes

Tracer titration

KD_app:

306 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

6 nM

raw data

Comment: MET(Y1230H)-NLuc

Reference: source

: 2.68

Z’: 0.86

: yes

Tracer titration

KD_app:

858 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

4 nM

raw data

Comment: MET(Y1235D)-NLuc

Reference: source

: 2.53

Z’: 0.86

: yes

Tracer titration

KD_app:

672 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

73 nM

raw data

Comment: NLuc-MKNK2

Reference: source

: 4.37

Z’: 0.59

: yes

Tracer titration

KD_app:

449 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

1 µM

raw data

Comment: NLuc-MOK

Reference: source

: 2.52

Z’: 0.87

: yes

Tracer titration

KD_app:

1111 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

2 µM

raw data

Comment: MST1R-NLuc

Reference: source

: 4.62

Z’: 0.9

: yes

Tracer titration

KD_app:

568 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

17 nM

raw data

Comment: MUSK-NLuc

Reference: source

: 12.47

Z’: 0.92

: yes

Tracer titration

KD_app:

128 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

589 nM

raw data

Comment: MYLK2-NLuc

Reference: source

: 7.57

Z’: 0.95

: yes

Tracer titration

KD_app:

1282 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

240 nM

raw data

Comment: MYLK3-NLuc

Reference: source

: 4.92

Z’: 0.71

: yes

Tracer titration

KD_app:

218 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

14 nM

raw data

Comment: MYLK4-NLuc

Reference: source

: 3.44

Z’: 0.91

: yes

Tracer titration

KD_app:

1562 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

344 nM

raw data

Comment: NLuc-NEK1

Reference: source

: 3.43

Z’: 0.7

: yes

Tracer titration

KD_app:

692 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

323 nM

raw data

Comment: NLuc-NEK11

Reference: source

: 2.78

Z’: 0.76

: yes

Tracer titration

KD_app:

748 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

517 nM

raw data

Comment: NLuc-NEK2

Reference: source

: 5.76

Z’: 0.79

: yes

Tracer titration

KD_app:

768 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

561 nM

raw data

Comment: NLuc-NEK3

Reference: source

: 2.25

Z’: 0.81

: yes

Tracer titration

KD_app:

489 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

802 nM

raw data

Comment: NLuc-NEK4

Reference: source

: 6.37

Z’: 0.88

: yes

Tracer titration

KD_app:

84 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

238 nM

raw data

Comment: NLuc-NEK5

Reference: source

: 5.94

Z’: 0.79

: yes

Tracer titration

KD_app:

681 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

569 nM

raw data

Comment: NLuc-NEK9

Reference: source

: 6.19

Z’: 0.92

: yes

Tracer titration

KD_app:

318 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

30 nM

raw data

Comment: NIM1K-NLuc

Reference: source

: 2.47

Z’: 0.79

: yes

Tracer titration

KD_app:

1228 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

351 nM

raw data

Comment: NLK-NLuc

Reference: source

: 4.69

Z’: 0.94

: yes

Tracer titration

KD_app:

359 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

5 nM

raw data

Comment: NTRK1-NLuc

Reference: source

: 4.47

Z’: 0.73

: yes

Tracer titration

KD_app:

445 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

4 nM

raw data

Comment: NTRK2-NLuc

Reference: source

: 3.41

Z’: 0.74

: yes

Tracer titration

KD_app:

29 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

12 nM

raw data

Comment: NLuc-NUAK1

Reference: source

: 2.39

Z’: 0.77

: yes

Tracer titration

KD_app:

193 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

6 nM

raw data

Comment: NUAK2-NLuc

Reference: source

: 7.61

Z’: 0.94

: yes

Tracer titration

KD_app:

585 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

359 nM

raw data

Comment: PAK4-NLuc

Reference: source

: 3.48

Z’: 0.89

: yes

Tracer titration

KD_app:

377 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

38 nM

raw data

Comment: PAK6-NLuc

Reference: source

: 5.29

Z’: 0.93

: yes

Tracer titration

KD_app:

441 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

1 nM

raw data

Comment: NLuc-PHKG1

Reference: source

: 2.25

Z’: 0.81

: yes

Tracer titration

KD_app:

566 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

6 nM

raw data

Comment: NLuc-PHKG2

Reference: source

: 2.1

Z’: 0.9

: yes

Tracer titration

KD_app:

657 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 nM

raw data

Comment: NLuc-PIM3

Reference: source

: 2.48

Z’: 0.89

: yes

Tracer titration

KD_app:

374 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

240 nM

raw data

Comment: NLuc-PLK2

Reference: source

: 2.97

Z’: 0.85

: yes

Tracer titration

KD_app:

257 nM

raw data

Tracer displacement

i

Compound used for displacement:

BI-2536

EC₅₀:

10 nM

raw data

Comment: NLuc-PLK3

Reference: source

: 4.91

Z’: 0.85

: yes

Tracer titration

KD_app:

370 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

19 nM

raw data

Comment: NLuc-PLK4

Reference: source

: 4.85

Z’: 0.5

: yes

Tracer titration

KD_app:

267 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: NLuc-PRKAA1

Reference: source

: 5.32

Z’: 0.76

: yes

Tracer titration

KD_app:

106 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

6 nM

raw data

Comment: NLuc-PRKAA2

Reference: source

: 5.4

Z’: 0.89

: yes

Tracer titration

KD_app:

425 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

286 nM

raw data

Comment: PRKACA-NLuc

Reference: source

: 2.19

Z’: 0.82

: yes

Tracer titration

KD_app:

361 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

144 nM

raw data

Comment: PRKACB-NLuc

Reference: source

: 2.61

Z’: 0.58

: yes

Tracer titration

KD_app:

633 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

114 nM

raw data

Comment: PRKCE-NLuc

Reference: source

: 3.11

Z’: 0.89

: yes

Tracer titration

KD_app:

300 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

376 nM

raw data

Comment: PRKX-NLuc

Reference: source

: 3.74

Z’: 0.83

: yes

Tracer titration

KD_app:

130 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

24 nM

raw data

Comment: NLuc-PTK2

Reference: source

: 2.6

Z’: 0.91

: yes

Tracer titration

KD_app:

119 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

239 nM

raw data

Comment: PTK2B-NLuc

Reference: source

: 3.15

Z’: 0.89

: yes

Tracer titration

KD_app:

126 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

177 nM

raw data

Comment: PTK6-NLuc

Reference: source

: 4.27

Z’: 0.71

: yes

Tracer titration

KD_app:

231 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

16 nM

raw data

Comment: RET-NLuc

Reference: source

: 4.45

Z’: 0.85

: yes

Tracer titration

KD_app:

405 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

27 nM

raw data

Comment: RET(M918T)-NLuc

Reference: source

: 4.72

Z’: 0.75

: yes

Tracer titration

KD_app:

203 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: RET(V804L)-NLuc

Reference: source

: 5.07

Z’: 0.77

: yes

Tracer titration

KD_app:

185 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

5 nM

raw data

Comment: RET(V804M)-NLuc

Reference: source

: 4.74

Z’: 0.87

: yes

Tracer titration

KD_app:

962 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

4 µM

raw data

Comment: NLuc-RIOK2

Reference: source

: 2.24

Z’: 0.86

: yes

Tracer titration

KD_app:

598 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

588 nM

raw data

Comment: NLuc-RIPK1

Reference: source

: 4.73

Z’: 0.73

: yes

Tracer titration

KD_app:

544 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

14 nM

raw data

Comment: NLuc-RIPK2

Reference: source

: 5.53

Z’: 0.83

: yes

Tracer titration

KD_app:

68 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 µM

raw data

Comment: NLuc-RPS6KA1

Reference: source

: 3.46

Z’: 0.85

: yes

Tracer titration

KD_app:

125 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 µM

raw data

Comment: NLuc-RPS6KA2

Reference: source

: 3.6

Z’: 0.75

: yes

Tracer titration

KD_app:

35 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

517 nM

raw data

Comment: NLuc-RPS6KA3

: 3.46

Z’: 0.82

: yes

Tracer titration

KD_app:

42 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

366 nM

raw data

Comment: NLuc-RPS6KA3(I416V)

Reference: source

: 3.46

Z’: 0.89

: yes

Tracer titration

KD_app:

35 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

889 nM

raw data

Comment: NLuc-RPS6KA3(L608F)

Reference: source

: 4.52

Z’: 0.87

: yes

Tracer titration

KD_app:

69 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

388 nM

raw data

Comment: NLuc-RPS6KA4

Reference: source

: 3.06

Z’: 0.7

: yes

Tracer titration

KD_app:

137 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

43 nM

raw data

Comment: NLuc-RPS6KA6

Reference: source

: 5.85

Z’: 0.95

: yes

Tracer titration

KD_app:

245 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

24 nM

raw data

Comment: NLuc-SBK3

Reference: source

: 3.24

Z’: 0.87

: yes

Tracer titration

KD_app:

471 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

151 nM

raw data

Comment: SGK1-NLuc

Reference: source

: 6.16

Z’: 0.78

: yes

Tracer titration

KD_app:

117 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

1 nM

raw data

Comment: NLuc-SIK1

Reference: source

: 12.95

Z’: 0.78

: yes

Tracer titration

KD_app:

27 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

25 nM

raw data

Comment: NLuc-SNF1LK2

Reference: source

: 6.73

Z’: 0.95

: yes

Tracer titration

KD_app:

518 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: NLuc-SIK3(59–1321)

Reference: source

: 2.75

Z’: 0.81

: yes

Tracer titration

KD_app:

263 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 nM

raw data

Comment: SIK3-NLuc

: 2.4

Z’: 0.61

: yes

Tracer titration

KD_app:

720 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

17 nM

raw data

Comment: NLuc-SLK

Reference: source

: 3.04

Z’: 0.89

: yes

Tracer titration

KD_app:

590 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

205 nM

raw data

Comment: NLuc-SNRK

Reference: source

: 2.47

Z’: 0.92

: yes

Tracer titration

KD_app:

491 nM

raw data

Tracer displacement

i

Compound used for displacement:

Vemurafenib

EC₅₀:

760 nM

raw data

Comment: NLuc-SRMS

Reference: source

: 3.02

Z’: 0.65

: yes

Tracer titration

KD_app:

448 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

0 nM

raw data

Comment: NLuc-STK10

Reference: source

: 4.26

Z’: 0.91

: yes

Tracer titration

KD_app:

380 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

611 nM

raw data

Comment: NLuc-STK11

Reference: source

: 6.64

Z’: 0.7

: yes

Tracer titration

KD_app:

141 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

50 nM

raw data

Comment: NLuc-STK16

Reference: source

: 2.35

Z’: 0.53

: yes

Tracer titration

KD_app:

738 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

83 nM

raw data

Comment: STK17B-NLuc

Reference: source

: 2.92

Z’: 0.72

: yes

Tracer titration

KD_app:

275 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

315 nM

raw data

Comment: NLuc-STK3

Reference: source

: 2.47

Z’: 0.88

: yes

Tracer titration

KD_app:

285 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

187 nM

raw data

Comment: NLuc-STK32B

Reference: source

: 2.79

Z’: 0.73

: yes

Tracer titration

KD_app:

153 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

405 nM

raw data

Comment: NLuc-STK33

Reference: source

: 3.03

Z’: 0.94

: yes

Tracer titration

KD_app:

570 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

EC₅₀:

26 nM

raw data

Comment: STK35-NLuc

Reference: source

: 4.56

Z’: 0.82

: yes

Tracer titration

KD_app:

572 nM

raw data

Tracer displacement

i

Compound used for displacement:

HG-9-91-01

EC₅₀:

269 nM

raw data

Comment: NLuc-STK36

Reference: source

: 4.27

Z’: 0.96

: yes

Tracer titration

KD_app:

1328 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

333 nM

raw data

Comment: STK38-NLuc

Reference: source

: 4.12

Z’: 0.83

: yes

Tracer titration

KD_app:

671 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

4 µM

raw data

Comment: STK38L-NLuc

Reference: source

: 3.65

Z’: 0.79

: yes

Tracer titration

KD_app:

126 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

42 nM

raw data

Comment: NLuc-STK4

Reference: source

: 5.12

Z’: 0.81

: yes

Tracer titration

KD_app:

153 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

27 nM

raw data

Comment: NLuc-TBK1

Reference: source

: 4.32

Z’: 0.89

: yes

Tracer titration

KD_app:

403 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

308 nM

raw data

Comment: NLuc-TEC

Reference: source

: 5.74

Z’: 0.72

: yes

Tracer titration

KD_app:

502 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

24 nM

raw data

Comment: TEK-NLuc

Reference: source

: 15.33

Z’: 0.86

: yes

Tracer titration

KD_app:

438 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

66 nM

raw data

Comment: TEK(A1124V)-NLuc

Reference: source

: 18.35

Z’: 0.89

: yes

Tracer titration

KD_app:

278 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

114 nM

raw data

Comment: TEK(P883A)-NLuc

Reference: source

: 23.14

Z’: 0.88

: yes

Tracer titration

KD_app:

330 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

71 nM

raw data

Comment: TEK(R849W)-NLuc

Reference: source

: 18.14

Z’: 0.95

: yes

Tracer titration

KD_app:

244 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

105 nM

raw data

Comment: TEK(Y1108F)-NLuc

Reference: source

: 15.6

Z’: 0.84

: yes

Tracer titration

KD_app:

355 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

111 nM

raw data

Comment: TEK(Y897C)-NLuc

Reference: source

: 14.59

Z’: 0.98

: yes

Tracer titration

KD_app:

352 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

192 nM

raw data

Comment: TEK(Y897S)-NLuc

Reference: source

: 5.18

Z’: 0.71

: yes

Tracer titration

KD_app:

436 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

281 nM

raw data

Comment: TIE1-NLuc

Reference: source

: 2.77

Z’: 0.94

: yes

Tracer titration

KD_app:

252 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

540 nM

raw data

Comment: TLK1-NLuc

Reference: source

: 3.3

Z’: 0.94

: yes

Tracer titration

KD_app:

259 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

614 nM

raw data

Comment: TLK2-NLuc

Reference: source

: 5.01

Z’: 0.76

: yes

Tracer titration

KD_app:

36 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

30 nM

raw data

Comment: NLuc-TNK1

Reference: source

: 6.04

Z’: 0.82

: yes

Tracer titration

KD_app:

149 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

136 nM

raw data

Comment: TNK2-NLuc

Reference: source

: 3.2

Z’: 0.92

: yes

Tracer titration

KD_app:

404 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

16 nM

raw data

Comment: TNNI3K-NLuc

Reference: source

: 2.46

Z’: 0.89

: yes

Tracer titration

KD_app:

660 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

7 nM

raw data

Comment: TXK-NLuc

Reference: source

: 2.92

Z’: 0.94

: yes

Tracer titration

KD_app:

934 nM

raw data

Tracer displacement

i

Compound used for displacement:

Pacritinib

EC₅₀:

2 µM

raw data

Comment: TYK2-NLuc

Reference: source

: 2.14

Z’: 0.88

: yes

Tracer titration

KD_app:

3501 nM

raw data

Tracer displacement

i

Compound used for displacement:

Cerdulatinib

EC₅₀:

113 nM

raw data

Comment: TYK2(897–1187)-NLuc

Reference: source

: 4.63

Z’: 0.94

: yes

Tracer titration

KD_app:

1587 nM

raw data

Tracer displacement

i

Compound used for displacement:

Cerdulatinib

EC₅₀:

26 nM

raw data

Comment: TYK2(589–875)-NLuc

Reference: source

: 2.22

Z’: 0.9

: yes

Tracer titration

KD_app:

340 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

11 nM

raw data

Comment: TYRO3-NLuc

Reference: source

: 10.4

Z’: 0.8

: yes

Tracer titration

KD_app:

237 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

114 nM

raw data

Comment: NLuc-ULK1

Reference: source

: 4.69

Z’: 0.83

: yes

Tracer titration

KD_app:

951 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

28 nM

raw data

Comment: NLuc-ULK2

Reference: source

: 16.67

Z’: 0.78

: yes

Tracer titration

KD_app:

358 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

226 nM

raw data

Comment: NLuc-ULK3

Reference: source

: 3.53

Z’: 0.76

: yes

Tracer titration

KD_app:

178 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

12 nM

raw data

Comment: WEE1-NLuc

Reference: source

: 2.46

Z’: 0.89

: yes

Tracer titration

KD_app:

272 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

78 nM

raw data

Comment: NLuc-WEE2

Reference: source