|T000041, K5 Tracer

Compound Image

T000041

K5 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

: 5.07

Z’: 0.61

: yes

Tracer titration

KD_app:

276 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

4 nM

raw data

Comment: NLuc-AAK1

Reference: source

: 2.66

Z’: 0.88

: yes

Tracer titration

KD_app:

6299 nM

raw data

Tracer displacement

i

Compound used for displacement:

GSK-690693

EC₅₀:

276 nM

raw data

Comment: AKT2-NLuc

Reference: source

: 6.95

Z’: 0.95

: yes

Tracer titration

KD_app:

22 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

19 nM

raw data

Comment: AURKA-NLuc

Reference: source

: 11.59

Z’: 0.92

: yes

Tracer titration

KD_app:

6 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

5 nM

raw data

Comment: AURKB-NLuc

Reference: source

: 11.73

Z’: 0.82

: yes

Tracer titration

KD_app:

10 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

4 nM

raw data

Comment: AURKC-NLuc

Reference: source

: 3.03

Z’: 0.88

: yes

Tracer titration

KD_app:

76 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

8 nM

raw data

Comment: AXL-NLuc

Reference: source

: 3.71

Z’: 0.92

: yes

Tracer titration

KD_app:

607 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

146 nM

raw data

Comment: NLuc-BMP2K

Reference: source

: 3.23

Z’: 0.755

: yes

Tracer titration

KD_app:

501 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

23 nM

raw data

Comment: NLuc-BRSK2

Reference: source

: 5.71

Z’: 0.82

: yes

Tracer titration

KD_app:

292 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

461 nM

raw data

Comment: BTK-NLuc

Reference: source

: 5.23

Z’: 0.76

: yes

Tracer titration

KD_app:

171 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

4 nM

raw data

Comment: NLuc-CLK1

Reference: source

: 6.23

Z’: 0.95

: yes

Tracer titration

KD_app:

182 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

92 nM

raw data

Comment: CLK2-NLuc

Reference: source

: 2.95

Z’: 0.77

: yes

Tracer titration

KD_app:

171 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

12 nM

raw data

Comment: CLK4-NLuc

Reference: source

: 3.2

Z’: 0.7

: yes

Tracer titration

KD_app:

429 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

220 nM

raw data

Comment: CSNK2A2-NLuc

Reference: source

: 3.52

Z’: 0.79

: yes

Tracer titration

KD_app:

225 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

6 nM

raw data

Comment: EPHA6-NLuc

Reference: source

: 3.4

Z’: 0.88

: yes

Tracer titration

KD_app:

271 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

53 nM

raw data

Comment: EPHA7-NLuc

Reference: source

: 2.26

Z’: 0.85

: yes

Tracer titration

KD_app:

262 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

25 nM

raw data

Comment: FER-NLuc

Reference: source

: 3.0

Z’: 0.73

: yes

Tracer titration

KD_app:

284 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

16 nM

raw data

Comment: FGFR1-NLuc

Reference: source

: 2.44

Z’: 0.82

: yes

Tracer titration

KD_app:

265 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

917 nM

raw data

Comment: FGFR2-NLuc

Reference: source

: 2.73

Z’: 0.86

: yes

Tracer titration

KD_app:

214 nM

raw data

Tracer displacement

i

Compound used for displacement:

PD-173955

EC₅₀:

128 nM

raw data

Comment: FGFR3-NLuc

Reference: source

: 2.13

Z’: 0.68

: yes

Tracer titration

KD_app:

293 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

50 nM

raw data

Comment: FGFR4-NLuc

Reference: source

: 2.55

Z’: 0.86

: yes

Tracer titration

KD_app:

185 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

38 nM

raw data

Comment: FLT3-NLuc

Reference: source

: 14.59

Z’: 0.83

: yes

Tracer titration

KD_app:

108 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

22 nM

raw data

Comment: NLuc-GAK

Reference: source

: 2.55

Z’: 0.58

: yes

Tracer titration

KD_app:

646 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

432 nM

raw data

Comment: NLuc-IKBKE

Reference: source

: 3.34

Z’: 0.75

: yes

Tracer titration

KD_app:

42 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

4 nM

raw data

Comment: NLuc-IRAK3

Reference: source

: 4.0

Z’: 0.86

: yes

Tracer titration

KD_app:

325 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

11 nM

raw data

Comment: IRAK4-NLuc

Reference: source

: 3.14

Z’: 0.84

: yes

Tracer titration

KD_app:

30 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

217 nM

raw data

Comment: NLuc-ITK

Reference: source

: 2.55

Z’: 0.89

: yes

Tracer titration

KD_app:

512 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

26 nM

raw data

Comment: JAK3-NLuc

Reference: source

: 3.64

Z’: 0.95

: yes

Tracer titration

KD_app:

267 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

353 nM

raw data

Comment: LATS1-NLuc

Reference: source

: 3.22

Z’: 0.9

: yes

Tracer titration

KD_app:

201 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

42 nM

raw data

Comment: LATS2-NLuc

Reference: source

: 2.67

Z’: 0.82

: yes

Tracer titration

KD_app:

283 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

21 nM

raw data

Comment: LTK-NLuc

: 2.38

Z’: 0.87

: yes

Tracer titration

KD_app:

268 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

43 nM

raw data

Comment: NLuc-MAP3K10

Reference: source

: 2.6

Z’: 0.66

: yes

Tracer titration

KD_app:

362 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

875 nM

raw data

Comment: NLuc-MAP3K11

Reference: source

: 2.28

Z’: 0.835

: yes

Tracer titration

KD_app:

360 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

1 µM

raw data

Comment: NLuc-MAP3K12

Reference: source

: 3.13

Z’: 0.87

: yes

Tracer titration

KD_app:

626 nM

raw data

Tracer displacement

i

Compound used for displacement:

PD-173955

EC₅₀:

112 nM

raw data

Comment: MAP3K4-NLuc

Reference: source

: 2.75

Z’: 0.75

: yes

Tracer titration

KD_app:

331 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: NLuc-MAP3K9

Reference: source

: 2.83

Z’: 0.84

: yes

Tracer titration

KD_app:

426 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

175 nM

raw data

Comment: NLuc-MAP4K1

Reference: source

: 2.57

Z’: 0.82

: yes

Tracer titration

KD_app:

180 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

EC₅₀:

56 nM

raw data

Comment: NLuc-MAP4K2

Reference: source

: 2.25

Z’: 0.85

: yes

Tracer titration

KD_app:

271 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

EC₅₀:

497 nM

raw data

Comment: NLuc-MAP4K3

Reference: source

: 1.65

Z’: 0.59

: yes

Tracer titration

KD_app:

358 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

1 µM

raw data

Comment: NLuc-MAPK1

Reference: source

: 2.25

Z’: 0.81

: yes

Tracer titration

KD_app:

347 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

1 µM

raw data

Comment: JNK3-NLuc

Reference: source

: 6.51

Z’: 0.78

: yes

Tracer titration

KD_app:

368 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

18 nM

raw data

Comment: NLuc-MAPK4

Reference: source

: 2.67

Z’: 0.68

: yes

Tracer titration

KD_app:

435 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

34 nM

raw data

Comment: NLuc-MAPK6

Reference: source

: 4.2

Z’: 0.61

: yes

Tracer titration

KD_app:

315 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

41 nM

raw data

Comment: NLuc-MAPK8

Reference: source

: 2.13

Z’: 0.65

: yes

Tracer titration

KD_app:

373 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 nM

raw data

Comment: NLuc-MARK2

Reference: source

: 3.24

Z’: 0.783

: yes

Tracer titration

KD_app:

430 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

3 nM

raw data

Comment: NLuc-MARK4

Reference: source

: 3.23

Z’: 0.95

: yes

Tracer titration

KD_app:

297 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

487 nM

raw data

Comment: NLuc-MELK

Reference: source

: 5.28

Z’: 0.723

: yes

Tracer titration

KD_app:

182 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

51 nM

raw data

Comment: MET-NLuc

Reference: source

: 4.79

Z’: 0.81

: yes

Tracer titration

KD_app:

212 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

94 nM

raw data

Comment: MUSK-NLuc

Reference: source

: 4.2

Z’: 0.91

: yes

Tracer titration

KD_app:

380 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

63 nM

raw data

Comment: MYLK2-NLuc

Reference: source

: 2.47

Z’: 0.67

: yes

Tracer titration

KD_app:

839 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

287 nM

raw data

Comment: NLuc-NEK3

Reference: source

: 2.25

Z’: 0.82

: yes

Tracer titration

KD_app:

426 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

EC₅₀:

124 nM

raw data

Comment: NLuc-NEK9

Reference: source

: 6.0

Z’: 0.85

: yes

Tracer titration

KD_app:

141 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

4 nM

raw data

Comment: NTRK1-NLuc

Reference: source

: 3.74

Z’: 0.79

: yes

Tracer titration

KD_app:

100 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

63 nM

raw data

Comment: NTRK2-NLuc

Reference: source

: 3.27

Z’: 0.94

: yes

Tracer titration

KD_app:

59 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

21 nM

raw data

Comment: NLuc-NUAK1

Reference: source

: 2.54

Z’: 0.83

: yes

Tracer titration

KD_app:

311 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

61 nM

raw data

Comment: PAK4-NLuc

Reference: source

: 2.51

Z’: 0.84

: yes

Tracer titration

KD_app:

323 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

38 nM

raw data

Comment: PAK5(PAK7)-NLuc

Reference: source

: 2.96

Z’: 0.93

: yes

Tracer titration

KD_app:

419 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

51 nM

raw data

Comment: NLuc-PHKG1

Reference: source

: 2.34

Z’: 0.92

: yes

Tracer titration

KD_app:

320 nM

raw data

Tracer displacement

i

Compound used for displacement:

PD-173955

EC₅₀:

60 nM

raw data

Comment: PKMYT1-NLuc

Reference: source

: 4.69

Z’: 0.84

: yes

Tracer titration

KD_app:

144 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

280 nM

raw data

Comment: NLuc-PLK4

Reference: source

: 5.76

Z’: 0.8

: yes

Tracer titration

KD_app:

179 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

216 nM

raw data

Comment: NLuc-PRKAA2

Reference: source

: 3.83

Z’: 0.86

: yes

Tracer titration

KD_app:

331 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

43 nM

raw data

Comment: PRKACA-NLuc

Reference: source

: 2.32

Z’: 0.5

: yes

Tracer titration

KD_app:

413 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

122 nM

raw data

Comment: PRKX-NLuc

Reference: source

: 2.69

Z’: 0.86

: yes

Tracer titration

KD_app:

81 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

21 nM

raw data

Comment: NLuc-PTK2

Reference: source

: 2.23

Z’: 0.81

: yes

Tracer titration

KD_app:

93 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

204 nM

raw data

Comment: PTK2B-NLuc

Reference: source

: 3.17

Z’: 0.86

: yes

Tracer titration

KD_app:

196 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

14 nM

raw data

Comment: RET-NLuc

Reference: source

: 4.81

Z’: 0.79

: yes

Tracer titration

KD_app:

337 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

1 µM

raw data

Comment: NLuc-RIOK2

Reference: source

: 4.9

Z’: 0.76

: yes

Tracer titration

KD_app:

112 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

19 nM

raw data

Comment: NLuc-RPS6KA1

Reference: source

: 4.12

Z’: 0.91

: yes

Tracer titration

KD_app:

122 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

58 nM

raw data

Comment: NLuc-RPS6KA2

Reference: source

: 4.83

Z’: 0.77

: yes

Tracer titration

KD_app:

75 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

21 nM

raw data

Comment: NLuc-RPS6KA3

Reference: source

: 4.45

Z’: 0.87

: yes

Tracer titration

KD_app:

105 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

37 nM

raw data

Comment: NLuc-RPS6KA4

Reference: source

: 3.18

Z’: 0.63

: yes

Tracer titration

KD_app:

299 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

28 nM

raw data

Comment: NLuc-RPS6KA6

Reference: source

: 2.5

Z’: 0.77

: yes

Tracer titration

KD_app:

546 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

594 nM

raw data

Comment: NLuc-STK11

Reference: source

: 10.2

Z’: 0.69

: yes

Tracer titration

KD_app:

167 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

37 nM

raw data

Comment: NLuc-STK16

Reference: source

: 3.48

Z’: 0.85

: yes

Tracer titration

KD_app:

257 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

4 µM

raw data

Comment: NLuc-STK32B

Reference: source

: 2.47

Z’: 0.85

: yes

Tracer titration

KD_app:

290 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

56 nM

raw data

Comment: NLuc-STK33

Reference: source

: 4.13

Z’: 0.85

: yes

Tracer titration

KD_app:

262 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

101 nM

raw data

Comment: NLuc-TBK1

Reference: source

: 7.14

Z’: 0.72

: yes

Tracer titration

KD_app:

273 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

29 nM

raw data

Comment: TEK-NLuc

Reference: source

: 4.18

Z’: 0.8

: yes

Tracer titration

KD_app:

205 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

369 nM

raw data

Comment: TIE1-NLuc

Reference: source

: 4.16

Z’: 0.74

: yes

Tracer titration

KD_app:

35 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

22 nM

raw data

Comment: NLuc-TNK1

Reference: source

: 4.0

Z’: 0.64

: yes

Tracer titration

KD_app:

321 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

22 nM

raw data

Comment: NLuc-ULK1

Reference: source

: 3.39

Z’: 0.87

: yes

Tracer titration

KD_app:

920 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

199 nM

raw data

Comment: NLuc-ULK2

Reference: source

: 4.29

Z’: 0.95

: yes

Tracer titration

KD_app:

47 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

EC₅₀:

18 nM

raw data

Comment: WEE1-NLuc

Reference: source