|T000041, K5 Tracer

Compound Image

T000041

K5 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 5.07

\(Z'\) 0.61

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

276 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: NLuc-AAK1

Reference: source

Assay window 2.66

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

6299 nM

raw data

Tracer displacement

i

Compound used for displacement:

GSK-690693

\(\mathrm{EC}_{50}\):

276 nM

raw data

Comment: AKT2-NLuc

Reference: source

Assay window 6.95

\(Z'\) 0.95

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

22 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

19 nM

raw data

Comment: AURKA-NLuc

Reference: source

Assay window 11.59

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

6 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

5 nM

raw data

Comment: AURKB-NLuc

Reference: source

Assay window 11.73

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

10 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: AURKC-NLuc

Reference: source

Assay window 3.03

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

76 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

8 nM

raw data

Comment: AXL-NLuc

Reference: source

Assay window 3.71

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

607 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

146 nM

raw data

Comment: NLuc-BMP2K

Reference: source

Assay window 3.23

\(Z'\) 0.755

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

501 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

23 nM

raw data

Comment: NLuc-BRSK2

Reference: source

Assay window 5.71

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

292 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

461 nM

raw data

Comment: BTK-NLuc

Reference: source

Assay window 5.23

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

171 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: NLuc-CLK1

Reference: source

Assay window 6.23

\(Z'\) 0.95

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

182 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

92 nM

raw data

Comment: CLK2-NLuc

Reference: source

Assay window 2.95

\(Z'\) 0.77

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

171 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

12 nM

raw data

Comment: CLK4-NLuc

Reference: source

Assay window 3.2

\(Z'\) 0.7

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

429 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

220 nM

raw data

Comment: CSNK2A2-NLuc

Reference: source

Assay window 3.52

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

225 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

6 nM

raw data

Comment: EPHA6-NLuc

Reference: source

Assay window 3.4

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

271 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

53 nM

raw data

Comment: EPHA7-NLuc

Reference: source

Assay window 2.26

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

262 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

25 nM

raw data

Comment: FER-NLuc

Reference: source

Assay window 3.0

\(Z'\) 0.73

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

284 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

16 nM

raw data

Comment: FGFR1-NLuc

Reference: source

Assay window 2.44

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

265 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

917 nM

raw data

Comment: FGFR2-NLuc

Reference: source

Assay window 2.73

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

214 nM

raw data

Tracer displacement

i

Compound used for displacement:

PD-173955

\(\mathrm{EC}_{50}\):

128 nM

raw data

Comment: FGFR3-NLuc

Reference: source

Assay window 2.13

\(Z'\) 0.68

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

293 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

50 nM

raw data

Comment: FGFR4-NLuc

Reference: source

Assay window 2.55

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

185 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

38 nM

raw data

Comment: FLT3-NLuc

Reference: source

Assay window 14.59

\(Z'\) 0.83

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

108 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

22 nM

raw data

Comment: NLuc-GAK

Reference: source

Assay window 2.55

\(Z'\) 0.58

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

646 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

432 nM

raw data

Comment: NLuc-IKBKE

Reference: source

Assay window 3.34

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

42 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: NLuc-IRAK3

Reference: source

Assay window 4.0

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

325 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

11 nM

raw data

Comment: IRAK4-NLuc

Reference: source

Assay window 3.14

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

30 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

217 nM

raw data

Comment: NLuc-ITK

Reference: source

Assay window 2.55

\(Z'\) 0.89

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

512 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

26 nM

raw data

Comment: JAK3-NLuc

Reference: source

Assay window 3.64

\(Z'\) 0.95

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

267 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

353 nM

raw data

Comment: LATS1-NLuc

Reference: source

Assay window 3.22

\(Z'\) 0.9

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

201 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

42 nM

raw data

Comment: LATS2-NLuc

Reference: source

Assay window 2.67

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

283 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

21 nM

raw data

Comment: LTK-NLuc

Assay window 2.38

\(Z'\) 0.87

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

268 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

43 nM

raw data

Comment: NLuc-MAP3K10

Reference: source

Assay window 2.6

\(Z'\) 0.66

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

362 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

875 nM

raw data

Comment: NLuc-MAP3K11

Reference: source

Assay window 2.28

\(Z'\) 0.835

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

360 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

1 µM

raw data

Comment: NLuc-MAP3K12

Reference: source

Assay window 3.13

\(Z'\) 0.87

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

626 nM

raw data

Tracer displacement

i

Compound used for displacement:

PD-173955

\(\mathrm{EC}_{50}\):

112 nM

raw data

Comment: MAP3K4-NLuc

Reference: source

Assay window 2.75

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

331 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: NLuc-MAP3K9

Reference: source

Assay window 2.83

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

426 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

175 nM

raw data

Comment: NLuc-MAP4K1

Reference: source

Assay window 2.57

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

180 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

\(\mathrm{EC}_{50}\):

56 nM

raw data

Comment: NLuc-MAP4K2

Reference: source

Assay window 2.25

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

271 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

\(\mathrm{EC}_{50}\):

497 nM

raw data

Comment: NLuc-MAP4K3

Reference: source

Assay window 1.65

\(Z'\) 0.59

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

358 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

1 µM

raw data

Comment: NLuc-MAPK1

Reference: source

Assay window 2.25

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

347 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

1 µM

raw data

Comment: JNK3-NLuc

Reference: source

Assay window 6.51

\(Z'\) 0.78

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

368 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

\(\mathrm{EC}_{50}\):

18 nM

raw data

Comment: NLuc-MAPK4

Reference: source

Assay window 2.67

\(Z'\) 0.68

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

435 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

34 nM

raw data

Comment: NLuc-MAPK6

Reference: source

Assay window 4.2

\(Z'\) 0.61

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

315 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

\(\mathrm{EC}_{50}\):

41 nM

raw data

Comment: NLuc-MAPK8

Reference: source

Assay window 2.13

\(Z'\) 0.65

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

373 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

3 nM

raw data

Comment: NLuc-MARK2

Reference: source

Assay window 3.24

\(Z'\) 0.783

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

430 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

3 nM

raw data

Comment: NLuc-MARK4

Reference: source

Assay window 3.23

\(Z'\) 0.95

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

297 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

\(\mathrm{EC}_{50}\):

487 nM

raw data

Comment: NLuc-MELK

Reference: source

Assay window 5.28

\(Z'\) 0.723

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

182 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

51 nM

raw data

Comment: MET-NLuc

Reference: source

Assay window 4.79

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

212 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

94 nM

raw data

Comment: MUSK-NLuc

Reference: source

Assay window 4.2

\(Z'\) 0.91

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

380 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

63 nM

raw data

Comment: MYLK2-NLuc

Reference: source

Assay window 2.47

\(Z'\) 0.67

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

839 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

\(\mathrm{EC}_{50}\):

287 nM

raw data

Comment: NLuc-NEK3

Reference: source

Assay window 2.25

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

426 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

124 nM

raw data

Comment: NLuc-NEK9

Reference: source

Assay window 6.0

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

141 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: NTRK1-NLuc

Reference: source

Assay window 3.74

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

100 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

63 nM

raw data

Comment: NTRK2-NLuc

Reference: source

Assay window 3.27

\(Z'\) 0.94

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

59 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

21 nM

raw data

Comment: NLuc-NUAK1

Reference: source

Assay window 2.54

\(Z'\) 0.83

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

311 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

61 nM

raw data

Comment: PAK4-NLuc

Reference: source

Assay window 2.51

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

323 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

38 nM

raw data

Comment: PAK5(PAK7)-NLuc

Reference: source

Assay window 2.96

\(Z'\) 0.93

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

419 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

51 nM

raw data

Comment: NLuc-PHKG1

Reference: source

Assay window 2.34

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

320 nM

raw data

Tracer displacement

i

Compound used for displacement:

PD-173955

\(\mathrm{EC}_{50}\):

60 nM

raw data

Comment: PKMYT1-NLuc

Reference: source

Assay window 4.69

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

144 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

280 nM

raw data

Comment: NLuc-PLK4

Reference: source

Assay window 5.76

\(Z'\) 0.8

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

179 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

216 nM

raw data

Comment: NLuc-PRKAA2

Reference: source

Assay window 3.83

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

331 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

43 nM

raw data

Comment: PRKACA-NLuc

Reference: source

Assay window 2.32

\(Z'\) 0.5

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

413 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

122 nM

raw data

Comment: PRKX-NLuc

Reference: source

Assay window 2.69

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

81 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

21 nM

raw data

Comment: NLuc-PTK2

Reference: source

Assay window 2.23

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

93 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

204 nM

raw data

Comment: PTK2B-NLuc

Reference: source

Assay window 3.17

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

196 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

14 nM

raw data

Comment: RET-NLuc

Reference: source

Assay window 4.81

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

337 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

1 µM

raw data

Comment: NLuc-RIOK2

Reference: source

Assay window 4.9

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

112 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

19 nM

raw data

Comment: NLuc-RPS6KA1

Reference: source

Assay window 4.12

\(Z'\) 0.91

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

122 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

58 nM

raw data

Comment: NLuc-RPS6KA2

Reference: source

Assay window 4.83

\(Z'\) 0.77

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

75 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

21 nM

raw data

Comment: NLuc-RPS6KA3

Reference: source

Assay window 4.45

\(Z'\) 0.87

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

105 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

37 nM

raw data

Comment: NLuc-RPS6KA4

Reference: source

Assay window 3.18

\(Z'\) 0.63

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

299 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

28 nM

raw data

Comment: NLuc-RPS6KA6

Reference: source

Assay window 2.5

\(Z'\) 0.77

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

546 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

594 nM

raw data

Comment: NLuc-STK11

Reference: source

Assay window 10.2

\(Z'\) 0.69

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

167 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

37 nM

raw data

Comment: NLuc-STK16

Reference: source

Assay window 3.48

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

257 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

4 µM

raw data

Comment: NLuc-STK32B

Reference: source

Assay window 2.47

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

290 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

56 nM

raw data

Comment: NLuc-STK33

Reference: source

Assay window 4.13

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

262 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

101 nM

raw data

Comment: NLuc-TBK1

Reference: source

Assay window 7.14

\(Z'\) 0.72

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

273 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

29 nM

raw data

Comment: TEK-NLuc

Reference: source

Assay window 4.18

\(Z'\) 0.8

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

205 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

369 nM

raw data

Comment: TIE1-NLuc

Reference: source

Assay window 4.16

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

35 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

22 nM

raw data

Comment: NLuc-TNK1

Reference: source

Assay window 4.0

\(Z'\) 0.64

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

321 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

22 nM

raw data

Comment: NLuc-ULK1

Reference: source

Assay window 3.39

\(Z'\) 0.87

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

920 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

199 nM

raw data

Comment: NLuc-ULK2

Reference: source

Assay window 4.29

\(Z'\) 0.95

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

47 nM

raw data

Tracer displacement

i

Compound used for displacement:

CTx-0294885

\(\mathrm{EC}_{50}\):

18 nM

raw data

Comment: WEE1-NLuc

Reference: source