|T000039, K11 Tracer

Compound Image

T000039

K11 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 3.52

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

220 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

51 nM

raw data

Comment: ACVR1-NLuc

Reference: source

Assay window 4.3

\(Z'\) 0.69

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

327 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

33 nM

raw data

Comment: ACVR1(G328V)

Reference: source

Assay window 3.62

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

520 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

129 nM

raw data

Comment: ACVR1(G356D)-NLuc

Reference: source

Assay window 3.79

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

402 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

51 nM

raw data

Comment: ACVR1(Q207D)

Reference: source

Assay window 3.59

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

388 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

41 nM

raw data

Comment: ACVR1(R206H)-NLuc

Reference: source

Assay window 3.31

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

184 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

378 nM

raw data

Comment: ACVRL1-NLuc

Reference: source

Assay window 2.25

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

252 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

113 nM

raw data

Comment: BMPR1A-NLuc

Reference: source

Assay window 3.09

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

231 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

34 nM

raw data

Comment: NLuc-CDKL1

Reference: source

Assay window 10.69

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

387 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

5 nM

raw data

Comment: NLuc-CDKL2

Reference: source

Assay window 7.81

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

196 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

5 nM

raw data

Comment: NLuc-CDKL3

Reference: source

Assay window 7.81

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

303 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: NLuc-CDKL5

Reference: source

Assay window 2.99

\(Z'\) 0.83

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

267 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

122 nM

raw data

Comment: EPHA3-NLuc

Reference: source

Assay window 2.13

\(Z'\) 0.87

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

33 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

524 nM

raw data

Comment: MAP2K6-NLuc

Reference: source

Assay window 2.17

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

105 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

8 µM

raw data

Comment: NLuc-NRK

Reference: source

Assay window 3.43

\(Z'\) 0.94

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

178 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

180 nM

raw data

Comment: TGFBR2-NLuc

Reference: source