|T000037, K4 Tracer

Compound Image

T000037

K4 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

: 2.7

Z’: 0.91

: yes

Tracer titration

KD_app:

63 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

3 nM

raw data

Comment: NLuc-ABL1

Reference: source

: 2.82

Z’: 0.63

: yes

Tracer titration

KD_app:

125 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

25 nM

raw data

Comment: NLuc-ABL1(E255K)

Reference: source

: 2.82

Z’: 0.53

: yes

Tracer titration

KD_app:

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

17 nM

raw data

Comment: NLuc-ABL1(F317I)

Reference: source

: 3.66

Z’: 0.65

: yes

Tracer titration

KD_app:

21 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

8 nM

raw data

Comment: NLuc-ABL1(H396P)

Reference: source

: 3.25

Z’: 0.9

: yes

Tracer titration

KD_app:

44 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

13 nM

raw data

Comment: NLuc-ABL1(M351T)

Reference: source

: 3.5

Z’: 0.92

: yes

Tracer titration

KD_app:

39 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

14 nM

raw data

Comment: NLuc-ABL1(Q252H)

Reference: source

: 3.09

Z’: 0.76

: yes

Tracer titration

KD_app:

43 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

15 nM

raw data

Comment: NLuc-ABL1(Y253F)

Reference: source

: 7.2

Z’: 0.81

: yes

Tracer titration

KD_app:

8 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

6 nM

raw data

Comment: NLuc-ABL2

Reference: source

: 4.83

Z’: 0.85

: yes

Tracer titration

KD_app:

139 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

3 nM

raw data

Comment: NLuc-BLK

Reference: source

: 6.95

Z’: 0.51

: yes

Tracer titration

KD_app:

262 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

3 nM

raw data

Comment: BMX-NLuc

Reference: source

: 2.35

Z’: 0.74

: yes

Tracer titration

KD_app:

111 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

8 nM

raw data

Comment: CSF1R-NLuc

Reference: source

: 3.76

Z’: 0.6

: yes

Tracer titration

KD_app:

611 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

18 nM

raw data

Comment: CSK-NLuc

Reference: source

: 9.62

Z’: 0.83

: yes

Tracer titration

KD_app:

22 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

2 nM

raw data

Comment: DDR1-NLuc

Reference: source

: 4.37

Z’: 0.71

: yes

Tracer titration

KD_app:

70 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

2 nM

raw data

Comment: DDR2-NLuc

Reference: source

: 4.32

Z’: 0.78

: yes

Tracer titration

KD_app:

62 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

3 nM

raw data

Comment: DDR2(N456S)-NLuc

Reference: source

: 4.05

Z’: 0.79

: yes

Tracer titration

KD_app:

177 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

3 nM

raw data

Comment: EPHA1-NLuc

Reference: source

: 5.92

Z’: 0.74

: yes

Tracer titration

KD_app:

41 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

2 nM

raw data

Comment: EPHA2-NLuc

Reference: source

: 8.37

Z’: 0.85

: yes

Tracer titration

KD_app:

28 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

1 nM

raw data

Comment: EPHA4-NLuc

Reference: source

: 6.97

Z’: 0.83

: yes

Tracer titration

KD_app:

23 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

1 nM

raw data

Comment: EPHA5-NLuc

Reference: source

: 9.03

Z’: 0.66

: yes

Tracer titration

KD_app:

7 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

2 nM

raw data

Comment: EPHA8-NLuc

Reference: source

: 6.1

Z’: 0.81

: yes

Tracer titration

KD_app:

13 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

4 nM

raw data

Comment: EPHB2-NLuc

Reference: source

: 4.47

Z’: 0.74

: yes

Tracer titration

KD_app:

87 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: EPHB3-NLuc

Reference: source

: 5.63

Z’: 0.74

: yes

Tracer titration

KD_app:

6 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

1 nM

raw data

Comment: EPHB4-NLuc

Reference: source

: 4.14

Z’: 0.83

: yes

Tracer titration

KD_app:

207 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

25 nM

raw data

Comment: NLuc-FGR

Reference: source

: 2.65

Z’: 0.72

: yes

Tracer titration

KD_app:

142 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

30 nM

raw data

Comment: FRK-NLuc

Reference: source

: 5.39

Z’: 0.84

: yes

Tracer titration

KD_app:

219 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

3 nM

raw data

Comment: FYN-NLuc

Reference: source

: 6.35

Z’: 0.87

: yes

Tracer titration

KD_app:

17 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

2 nM

raw data

Comment: FYN(Y531F)-NLuc

Reference: source

: 2.9

Z’: 0.61

: yes

Tracer titration

KD_app:

375 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

EC₅₀:

3 µM

raw data

Comment: HCK-NLuc

Reference: source

: 2.64

Z’: 0.62

: yes

Tracer titration

KD_app:

59 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

49 nM

raw data

Comment: KIT-NLuc

Reference: source

: 2.4

Z’: 0.64

: yes

Tracer titration

KD_app:

209 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

34 nM

raw data

Comment: KIT(A829P)-NLuc

Reference: source

: 2.22

Z’: 0.75

: yes

Tracer titration

KD_app:

327 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

62 nM

raw data

Comment: KIT(D816H)-NLuc

Reference: source

: 3.37

Z’: 0.85

: yes

Tracer titration

KD_app:

130 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

17 nM

raw data

Comment: KIT(L576P)-NLuc

Reference: source

: 4.35

Z’: 0.84

: yes

Tracer titration

KD_app:

24 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: KIT(V559D)-NLuc

Reference: source

: 2.25

Z’: 0.7

: yes

Tracer titration

KD_app:

358 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

117 nM

raw data

Comment: KIT (V559D,V654A)-NLuc

Reference: source

: 4.72

Z’: 0.86

: yes

Tracer titration

KD_app:

18 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

25 nM

raw data

Comment: LCK-NLuc

Reference: source

: 3.93

Z’: 0.8

: yes

Tracer titration

KD_app:

35 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

10 nM

raw data

Comment: LYN-NLuc

Reference: source

: 4.26

Z’: 0.8

: yes

Tracer titration

KD_app:

230 nM

raw data

Tracer displacement

i

Compound used for displacement:

Nilotinib

EC₅₀:

15 nM

raw data

Comment: NLuc-MAPK11

Reference: source

: 3.8

Z’: 0.63

: yes

Tracer titration

KD_app:

111 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

455 nM

raw data

Comment: MAPK14-NLuc

Reference: source

: 2.23

Z’: 0.78

: yes

Tracer titration

KD_app:

107 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

4 nM

raw data

Comment: PDGFRA(V561D)-NLuc

Reference: source

: 3.02

Z’: 0.63

: yes

Tracer titration

KD_app:

481 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

63 nM

raw data

Comment: PTK6-NLuc

Reference: source

: 3.47

Z’: 0.66

: yes

Tracer titration

KD_app:

112 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

1 nM

raw data

Comment: NLuc-RIPK2

Reference: source

: 6.92

Z’: 0.79

: yes

Tracer titration

KD_app:

118 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

5 nM

raw data

Comment: NLuc-SIK1

Reference: source

: 6.65

Z’: 0.76

: yes

Tracer titration

KD_app:

157 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

7 nM

raw data

Comment: NLuc-SIK2

Reference: source

: 4.0

Z’: 0.8

: yes

Tracer titration

KD_app:

549 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

72 nM

raw data

Comment: NLuc-SIK3

Reference: source

: 5.3

Z’: 0.74

: yes

Tracer titration

KD_app:

138 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

18 nM

raw data

Comment: SRC-NLuc

Reference: source

: 5.84

Z’: 0.92

: yes

Tracer titration

KD_app:

477 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

258 nM

raw data

Comment: NLuc-TEC

Reference: source

: 2.54

Z’: 0.74

: yes

Tracer titration

KD_app:

506 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

680 nM

raw data

Comment: NLuc-TESK1

Reference: source

: 3.89

Z’: 0.7

: yes

Tracer titration

KD_app:

145 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

19 nM

raw data

Comment: TXK-NLuc

Reference: source

: 2.64

Z’: 0.63

: yes

Tracer titration

KD_app:

345 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

24 nM

raw data

Comment: YES1-NLuc

Reference: source