|T000037, K4 Tracer

Compound Image

T000037

K4 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 2.7

\(Z'\) 0.91

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

63 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

3 nM

raw data

Comment: NLuc-ABL1

Reference: source

Assay window 2.82

\(Z'\) 0.63

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

125 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

25 nM

raw data

Comment: NLuc-ABL1(E255K)

Reference: source

Assay window 2.82

\(Z'\) 0.53

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

17 nM

raw data

Comment: NLuc-ABL1(F317I)

Reference: source

Assay window 3.66

\(Z'\) 0.65

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

21 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

8 nM

raw data

Comment: NLuc-ABL1(H396P)

Reference: source

Assay window 3.25

\(Z'\) 0.9

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

44 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

13 nM

raw data

Comment: NLuc-ABL1(M351T)

Reference: source

Assay window 3.5

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

39 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

14 nM

raw data

Comment: NLuc-ABL1(Q252H)

Reference: source

Assay window 3.09

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

43 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

15 nM

raw data

Comment: NLuc-ABL1(Y253F)

Reference: source

Assay window 7.2

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

8 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

6 nM

raw data

Comment: NLuc-ABL2

Reference: source

Assay window 4.83

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

139 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

3 nM

raw data

Comment: NLuc-BLK

Reference: source

Assay window 6.95

\(Z'\) 0.51

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

262 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

3 nM

raw data

Comment: BMX-NLuc

Reference: source

Assay window 2.35

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

111 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

8 nM

raw data

Comment: CSF1R-NLuc

Reference: source

Assay window 3.76

\(Z'\) 0.6

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

611 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

18 nM

raw data

Comment: CSK-NLuc

Reference: source

Assay window 9.62

\(Z'\) 0.83

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

22 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: DDR1-NLuc

Reference: source

Assay window 4.37

\(Z'\) 0.71

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

70 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: DDR2-NLuc

Reference: source

Assay window 4.32

\(Z'\) 0.78

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

62 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

3 nM

raw data

Comment: DDR2(N456S)-NLuc

Reference: source

Assay window 4.05

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

177 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

3 nM

raw data

Comment: EPHA1-NLuc

Reference: source

Assay window 5.92

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

41 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: EPHA2-NLuc

Reference: source

Assay window 8.37

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

28 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

1 nM

raw data

Comment: EPHA4-NLuc

Reference: source

Assay window 6.97

\(Z'\) 0.83

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

23 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

1 nM

raw data

Comment: EPHA5-NLuc

Reference: source

Assay window 9.03

\(Z'\) 0.66

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

7 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: EPHA8-NLuc

Reference: source

Assay window 6.1

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

13 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: EPHB2-NLuc

Reference: source

Assay window 4.47

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

87 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

5 nM

raw data

Comment: EPHB3-NLuc

Reference: source

Assay window 5.63

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

6 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

1 nM

raw data

Comment: EPHB4-NLuc

Reference: source

Assay window 4.14

\(Z'\) 0.83

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

207 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

25 nM

raw data

Comment: NLuc-FGR

Reference: source

Assay window 2.65

\(Z'\) 0.72

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

142 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

30 nM

raw data

Comment: FRK-NLuc

Reference: source

Assay window 5.39

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

219 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

3 nM

raw data

Comment: FYN-NLuc

Reference: source

Assay window 6.35

\(Z'\) 0.87

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

17 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: FYN(Y531F)-NLuc

Reference: source

Assay window 2.9

\(Z'\) 0.61

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

375 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bosutinib

\(\mathrm{EC}_{50}\):

3 µM

raw data

Comment: HCK-NLuc

Reference: source

Assay window 2.64

\(Z'\) 0.62

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

59 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

49 nM

raw data

Comment: KIT-NLuc

Reference: source

Assay window 2.4

\(Z'\) 0.64

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

209 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

34 nM

raw data

Comment: KIT(A829P)-NLuc

Reference: source

Assay window 2.22

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

327 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

62 nM

raw data

Comment: KIT(D816H)-NLuc

Reference: source

Assay window 3.37

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

130 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

17 nM

raw data

Comment: KIT(L576P)-NLuc

Reference: source

Assay window 4.35

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

24 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

5 nM

raw data

Comment: KIT(V559D)-NLuc

Reference: source

Assay window 2.25

\(Z'\) 0.7

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

358 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

117 nM

raw data

Comment: KIT (V559D,V654A)-NLuc

Reference: source

Assay window 4.72

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

18 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

25 nM

raw data

Comment: LCK-NLuc

Reference: source

Assay window 3.93

\(Z'\) 0.8

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

35 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

10 nM

raw data

Comment: LYN-NLuc

Reference: source

Assay window 4.26

\(Z'\) 0.8

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

230 nM

raw data

Tracer displacement

i

Compound used for displacement:

Nilotinib

\(\mathrm{EC}_{50}\):

15 nM

raw data

Comment: NLuc-MAPK11

Reference: source

Assay window 3.8

\(Z'\) 0.63

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

111 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

455 nM

raw data

Comment: MAPK14-NLuc

Reference: source

Assay window 2.23

\(Z'\) 0.78

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

107 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: PDGFRA(V561D)-NLuc

Reference: source

Assay window 3.02

\(Z'\) 0.63

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

481 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

63 nM

raw data

Comment: PTK6-NLuc

Reference: source

Assay window 3.47

\(Z'\) 0.66

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

112 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

1 nM

raw data

Comment: NLuc-RIPK2

Reference: source

Assay window 6.92

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

118 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

5 nM

raw data

Comment: NLuc-SIK1

Reference: source

Assay window 6.65

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

157 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

7 nM

raw data

Comment: NLuc-SIK2

Reference: source

Assay window 4.0

\(Z'\) 0.8

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

549 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

72 nM

raw data

Comment: NLuc-SIK3

Reference: source

Assay window 5.3

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

138 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

18 nM

raw data

Comment: SRC-NLuc

Reference: source

Assay window 5.84

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

477 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

258 nM

raw data

Comment: NLuc-TEC

Reference: source

Assay window 2.54

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

506 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

680 nM

raw data

Comment: NLuc-TESK1

Reference: source

Assay window 3.89

\(Z'\) 0.7

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

145 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

19 nM

raw data

Comment: TXK-NLuc

Reference: source

Assay window 2.64

\(Z'\) 0.63

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

345 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

24 nM

raw data

Comment: YES1-NLuc

Reference: source