|T000031, CUL3NTD

T000031

CUL3NTD

  • Description

    UniprotID:

    Q13618

    Dye:

    AF488
  • Properties

    Store at:

    -20 °C

    Absorption:

    475 nm

    Emission:

    520 nm
  • References

    Synthesis:

    Publication

    Contributing Lab:

    Mazitschek Lab, Massachusetts General Hospital, Boston

  • Experimental

    Assay window 33.0
    \(Z'\) 0.91
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    211 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    Cul3 (1-388)

    \(\mathrm{IC}_{50}\):

    259 nM
    displacement curve
    raw data

    Comment: 30 nM KEAP1(48–213), TR-FRET donor: CoraFluor-1

    Reference: PMID

    Assay window 56.2
    \(Z'\) 0.94
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    21 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    Cul3 (1-388)

    \(\mathrm{IC}_{50}\):

    24 nM
    displacement curve
    raw data

    Comment: 3 nM KLHL11 (67-340), TR-FRET donor: CoraFluor-1

    Reference: PMID