|T000026, NCP406 (JQ1-FITC)
Compound Image

T000026

NCP406 (JQ1-FITC)

  • Description

    Formula:

    C50H50ClN7O9S2

    avg. MolWt:

    992.58 u

    PAINS elements:

    none detected

    SMILES:

    display
  • Properties

    Store at:

    -20 °C

    Absorption:

    475 nm

    Emission:

    520 nm

    clogP:

    8.4
  • References

    Synthesis:

    Publication

    Contributing Lab:

    Mazitschek Lab, Massachusetts General Hospital, Boston

    Vendor:

    Tocris
  • Experimental

    Assay window 25.8
    \(Z'\) 0.87
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    6 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    JQ1

    \(\mathrm{IC}_{50}\):

    77 nM
    displacement curve
    raw data

    Comment: GST-BRD4(49-170), TR-FRET donor: CoraFluor-1

    Reference: PMID

    Assay window 20.5
    \(Z'\) 0.87
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    9 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    JQ1

    \(\mathrm{IC}_{50}\):

    100 nM
    displacement curve
    raw data

    Comment: FL-BRD4 from MCF7 lysates, TR-FRET donor: CoraFluor-1

    Reference: PMID

    Assay window 20.4
    \(Z'\) 0.83
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    6 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    JQ1

    \(\mathrm{IC}_{50}\):

    144 nM
    displacement curve
    raw data

    Comment: GST-BRD4(341-470), TR-FRET donor: CoraFluor-1

    Reference: PMID