|T000022, BRD Tracer-02 (CS1810C20)

T000022

BRD Tracer-02 (CS1810C20)

Description

Formula:

C₃₃H₃₃BF₂N₁₀O₅S

avg. MolWt:

730.57 u

PAINS elements:

none detected

SMILES:

display

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm

clogP:

4.07
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 4.37

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

251 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

725 nM

raw data

Comment: NLuc-BRD2

Reference: PMID

Assay window 3.65

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

588 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

2 µM

raw data

Comment: NLuc-BRD2(66-186)

Assay window 4.43

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

615 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

2 µM

raw data

Comment: NLuc-BRD2(332-453)

Assay window 5.59

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

359 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

531 nM

raw data

Comment: NLuc-BRD3

Reference: PMID

Assay window 9.88

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

523 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

647 nM

raw data

Comment: NLuc-BRD3(20-148)

Assay window 9.86

\(Z'\) 0.9

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

529 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

403 nM

raw data

Comment: NLuc-BRD3(299-420)

Assay window 5.62

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

293 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

283 nM

raw data

Comment: NLuc-BRD4

Reference: PMID

Assay window 7.5

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

555 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

93 nM

raw data

Comment: NLuc-BRD4(44-168)

Assay window 13.4

\(Z'\) 0.94

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

194 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

274 nM

raw data

Comment: NLuc-BRDT

Reference: PMID

Assay window 17.93

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

293 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

396 nM

raw data

Comment: NLuc-BRDT(13-139)

Assay window 1.5

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

168 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

378 nM

raw data

Comment: TAF1-NLuc

Assay window 4.5

\(Z'\) 0.87

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

198 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

203 nM

raw data

Comment: NLuc-TAF1-BD1-BD2 (1384-1638)

Assay window 7.9

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

196 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

263 nM

raw data

Comment: NLuc-TAF1-BD2 (1515-1669)

Assay window 1.7

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

271 nM

raw data

Tracer displacement

i

Compound used for displacement:

Bromosporine

\(\mathrm{EC}_{50}\):

1 µM

raw data

Comment: TAF1L-NLuc