|T000018, CRBN Tracer

Compound Image

T000018

CRBN Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 5.2

\(Z'\) 0.71

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

305 nM

raw data

Tracer displacement

i

Compound used for displacement:

Lenalidomide

\(\mathrm{EC}_{50}\):

112 nM

raw data

Comment: NLuc-CRBN + DDB1

Reference: source

Assay window 3.3

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}\):

87 nM

raw data

Tracer displacement

i

Compound used for displacement:

Lenalidomide

\(\mathrm{IC}_{50}\):

174 nM

raw data

Comment: NLuc-CRBN + DDB1

Reference: source