|T000017, K9 Tracer

Compound Image

T000017

K9 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

: 3.07

Z’: 0.94

: yes

Tracer titration

KD_app:

255 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

51 nM

raw data

Comment: NLuc-CAMK1

Reference: source

: 2.83

Z’: 0.88

: yes

Tracer titration

KD_app:

255 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

51 nM

raw data

Comment: NLuc-CAMK1D

Reference: source

: 3.29

Z’: 0.89

: yes

Tracer titration

KD_app:

316 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

EC₅₀:

832 nM

raw data

Comment: NLuc-CAMK1G

Reference: source

: 3.77

Z’: 0.82

: yes

Tracer titration

KD_app:

460 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

EC₅₀:

137 nM

raw data

Comment: CDK1-NLuc + CycB1

Reference: source

: 3.89

Z’: 0.74

: yes

Tracer titration

KD_app:

433 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

EC₅₀:

26 nM

raw data

Comment: CDK1-NLuc + CycE1

Reference: source

: 2.68

Z’: 0.74

: yes

Tracer titration

KD_app:

258 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

44 nM

raw data

Comment: NLuc-CHEK1

Reference: source

: 4.63

Z’: 0.96

: yes

Tracer titration

KD_app:

235 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

42 nM

raw data

Comment: CLK2-NLuc

Reference: source

: 3.45

Z’: 0.76

: yes

Tracer titration

KD_app:

132 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

36 nM

raw data

Comment: CLK4-NLuc

Reference: source

: 3.49

Z’: 0.95

: yes

Tracer titration

KD_app:

349 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

EC₅₀:

4 nM

raw data

Comment: EPHB1-NLuc

Reference: source

: 3.79

Z’: 0.88

: yes

Tracer titration

KD_app:

258 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

420 nM

raw data

Comment: FES-NLuc

Reference: source

: 2.44

Z’: 0.79

: yes

Tracer titration

KD_app:

83 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

30 nM

raw data

Comment: FLT1-NLuc

Reference: source

: 3.25

Z’: 0.73

: yes

Tracer titration

KD_app:

21 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

4 nM

raw data

Comment: FLT3(K663Q)-NLuc

Reference: source

: 3.15

Z’: 0.76

: yes

Tracer titration

KD_app:

181 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

182 nM

raw data

Comment: IGF1R-NLuc

Reference: source

: 3.27

Z’: 0.81

: yes

Tracer titration

KD_app:

150 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

394 nM

raw data

Comment: https://www.promega.de/products/cell-signaling/kinase-target-engagement/insr-nanoluc-fusion-vector/?catNum=NV3271

Reference: source

: 2.01

Z’: 0.82

: yes

Tracer titration

KD_app:

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

Pacritinib

EC₅₀:

2 µM

raw data

Comment: NLuc-IRAK1

Reference: source

: 3.97

Z’: 0.88

: yes

Tracer titration

KD_app:

138 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

87 nM

raw data

Comment: KIT(D816V)-NLuc

Reference: source

: 3.11

Z’: 0.68

: yes

Tracer titration

KD_app:

5 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: LRRK2-NLuc

: 2.42

Z’: 0.76

: yes

Tracer titration

KD_app:

4 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

1 nM

raw data

Comment: LRRK2(G2019S)-NLuc

Reference: source

: 3.52

Z’: 0.74

: yes

Tracer titration

KD_app:

20 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: LRRK2(I2020T)-NLuc

Reference: source

: 2.65

Z’: 0.65

: yes

Tracer titration

KD_app:

3 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

2 nM

raw data

Comment: LRRK2(R1441C)-NLuc

Reference: source

: 5.99

Z’: 0.73

: yes

Tracer titration

KD_app:

244 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

EC₅₀:

106 nM

raw data

Comment: LTK-NLuc

Reference: source

: 3.19

Z’: 0.92

: yes

Tracer titration

KD_app:

188 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

975 nM

raw data

Comment: NLuc-MAP3K12

Reference: source

: 2.11

Z’: 0.86

: yes

Tracer titration

KD_app:

43 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

1 µM

raw data

Comment: NLuc-MAP3K13

Reference: source

: 4.94

Z’: 0.79

: yes

Tracer titration

KD_app:

256 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

7 nM

raw data

Comment: NLuc-MARK2

Reference: source

: 2.93

Z’: 0.81

: yes

Tracer titration

KD_app:

175 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

932 nM

raw data

Comment: NLuc-MARK3

Reference: source

: 3.79

Z’: 0.88

: yes

Tracer titration

KD_app:

193 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

239 nM

raw data

Comment: NLuc-MELK

Reference: source

: 3.36

Z’: 0.86

: yes

Tracer titration

KD_app:

577 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

EC₅₀:

198 nM

raw data

Comment: NLuc-MELK(T460M)

Reference: source

: 2.51

Z’: 0.77

: yes

Tracer titration

KD_app:

91 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

239 nM

raw data

Comment: MERTK-NLuc

Reference: source

: 2.47

Z’: 0.84

: yes

Tracer titration

KD_app:

144 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

EC₅₀:

6 nM

raw data

Comment: MERTK(A708S)

Reference: source

: 4.05

Z’: 0.9

: yes

Tracer titration

KD_app:

259 nM

raw data

Tracer displacement

i

Compound used for displacement:

Crizotinib

EC₅₀:

339 nM

raw data

Comment: MST1R-NLuc

Reference: source

: 0.77

Z’: 0.77

: yes

Tracer titration

KD_app:

81 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

EC₅₀:

4 µM

raw data

Comment: NEK6-NLuc

Reference: source

: 2.3

Z’: 0.68

: yes

Tracer titration

KD_app:

200 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

1 nM

raw data

Comment: PAK1(M248-H545)-NLuc fusion

: 2.88

Z’: 0.68

: yes

Tracer titration

K_D:

225 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

IC₅₀:

0 nM

raw data

Comment: PAK1(M248-H545)-NLuc fusion

: 1.56

Z’: 0.85

: yes

Tracer titration

KD_app:

199 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

9 nM

raw data

Comment: NLuc-PAK2(D229-R524)

: 2.02

Z’: 0.84

: yes

Tracer titration

K_D:

131 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

IC₅₀:

4 nM

raw data

Comment: NLuc-PAK2(D229-R524)

: 2.28

Z’: 0.66

: yes

Tracer titration

K_D:

263 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

IC₅₀:

1 nM

raw data

Comment: PAK3(D263-R559)-NLuc

: 2.34

Z’: 0.79

: yes

Tracer titration

KD_app:

174 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

EC₅₀:

79 nM

raw data

Comment: PIK3C3-NLuc

Reference: source

: 4.29

Z’: 0.85

: yes

Tracer titration

KD_app:

148 nM

raw data

Tracer displacement

i

Compound used for displacement:

BI-2536

EC₅₀:

13 nM

raw data

Comment: NLuc-PLK2

Reference: source

: 5.17

Z’: 0.93

: yes

Tracer titration

KD_app:

327 nM

raw data

Tracer displacement

i

Compound used for displacement:

BI-2536

EC₅₀:

26 nM

raw data

Comment: NLuc-PLK3

Reference: source

: 3.02

Z’: 0.84

: yes

Tracer titration

KD_app:

209 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

157 nM

raw data

Comment: PRKG2-NLuc

Reference: source

: 3.52

Z’: 0.95

: yes

Tracer titration

KD_app:

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

KW-2449

EC₅₀:

323 nM

raw data

Comment: NLuc-RIPK1

Reference: source

: 2.0

Z’: 0.8

: yes

Tracer titration

KD_app:

81 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

EC₅₀:

3 µM

raw data

Comment: SGK2-NLuc

Reference: source

: 3.01

Z’: 0.77

: yes

Tracer titration

KD_app:

138 nM

raw data

Tracer displacement

i

Compound used for displacement:

KW-2449

EC₅₀:

546 nM

raw data

Comment: STK17B-NLuc

Reference: source

: 3.31

Z’: 0.86

: yes

Tracer titration

KD_app:

256 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

EC₅₀:

2 µM

raw data

Comment: STK24-NLuc

Reference: source

: 5.26

Z’: 0.69

: yes

Tracer titration

KD_app:

142 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

EC₅₀:

1 µM

raw data

Comment: STK26-NLuc

Reference: source

: 2.34

Z’: 0.9

: yes

Tracer titration

KD_app:

121 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

338 nM

raw data

Comment: NLuc-STK32A

Reference: source

: 5.61

Z’: 0.94

: yes

Tracer titration

KD_app:

251 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

EC₅₀:

46 nM

raw data

Comment: NLuc-STK32B

Reference: source

: 2.14

Z’: 0.72

: yes

Tracer titration

KD_app:

74 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

EC₅₀:

335 nM

raw data

Comment: NLuc-TSSK1B

Reference: source

: 3.54

Z’: 0.85

: yes

Tracer titration

KD_app:

183 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

EC₅₀:

2 µM

raw data

Comment: TTK-NLuc

Reference: source