|T000015, p62-LIR Tracer

T000015

p62-LIR Tracer

Description

Formula:

C₁₁₀H₁₅₅N₂₆O₃₅⁺

avg. MolWt:

2401.6 u

SMILES:

display

BILN:

S-D-N-S-S-G-G-D-D-D-W-T-H-L-S-S-K-K(Cy5)

Properties

Store at:

-20 °C

Absorption:

590 nm

Emission:

675 nm

clogP:

-8.77
References

Contributing Lab:

Knapp Lab, University of Frankfurt
Experimental

Assay window 1.9

\(Z'\) 0.6

CP agreement yes

Protein titration

\(K_\mathrm{D}\):

3443 nM

raw data

Tracer displacement

i

Compound used for displacement:

unlabelled p62-LIR peptide

\(\mathrm{IC}_{50}\):

18 µM

raw data

Comment: use 30 nM of the p62-LIR peptide tracer

Reference: source

Assay window 1.9

\(Z'\) 0.6

CP agreement yes

Protein titration

\(K_\mathrm{D}\):

2495 nM

raw data

Tracer displacement

i

Compound used for displacement:

unlabelled p62-LIR peptide

\(\mathrm{IC}_{50}\):

14 µM

raw data

Comment: use 30 nM of the p62-LIR peptide tracer

Reference: source

Assay window 1.5

\(Z'\) 0.5

CP agreement yes

Protein titration

\(K_\mathrm{D}\):

> 10 µM

raw data

Tracer displacement

i

Compound used for displacement:

unlabelled p62-LIR peptide

\(\mathrm{IC}_{50}\):

46 µM

raw data

Comment: use 30 nM of the p62-LIR peptide tracer

Reference: source

Assay window 1.5

\(Z'\) 0.8

CP agreement yes

Protein titration

\(K_\mathrm{D}\):

7687 nM

raw data

Tracer displacement

i

Compound used for displacement:

unlabelled p62-LIR peptide

\(\mathrm{IC}_{50}\):

17 µM

raw data

Comment: use 30 nM of the p62-LIR peptide tracer

Reference: source

Assay window 1.6

\(Z'\) 0.7

CP agreement yes

Protein titration

\(K_\mathrm{D}\):

5091 nM

raw data

Tracer displacement

i

Compound used for displacement:

unlabelled p62-LIR peptide

\(\mathrm{IC}_{50}\):

19 µM

raw data

Comment: use 30 nM of the p62-LIR peptide tracer

Reference: source

Assay window 1.5

\(Z'\) 0.6

CP agreement yes

Protein titration

\(K_\mathrm{D}\):

7279 nM

raw data

Tracer displacement

i

Compound used for displacement:

unlabelled p62-LIR peptide

\(\mathrm{IC}_{50}\):

32 µM

raw data

Comment: use 30 nM of the p62-LIR peptide tracer

Reference: source